Fig. 2.
Toxin A induces transcription of Bak. (A) NCM460 cells were incubated with toxin A (3 nM) for 0, 12, 24, or 36 h. Total RNA was isolated and the amounts of Bak transcripts were determined by quantitative RT-PCR and normalized against PPIA. (B) NCM460 cells were incubated with toxin A (3 nM) for 6 h, and Bak mRNA expression was determined by semi-quantitative RTPCR. The presented results are representative of three independent experiments.