Journal of Microbiology and Biotechnology

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Table. 1. Table 1

Primers used for fingerprinting of PKS and NRPS genes.

Type	Primer name	Target gene		Size (bp)	Annealing (°C)*	Reference
Type I PKS	K1F	PKS-I ketoacyl synthase (KS) domains and methyl malonyl transferase domains/PKS-I, KS-AT fragments	TSAAGTCSAACATCGGBCA		65	[10]
	M6R		CGCAGGTTSCSGTACCAGTA		65	[10]
	PKS-I-A	KS domains	GCSATGGAYCCSCARCARCGSVT	700	60	[21]
	PKS-I-B	KS domains	GTSCCSGTSCCRTGSSCYTCSAC	700	60	[21]
	KSMAF	Beta-KS domain	TSGCSATGGACCCSCAGCAG	~700	68	[22]
	KSMBR	Beta-KS domain	CCSGTSCCGTGSGCCTCSAC	~700	68	[22]
	KSI1f	Beta-ketosynthase domains	GCI ATGGAYCCICARCARMGIVT	700	50	[9]
	KSI2r	Beta-ketosynthase domains	GTICCIGTICCRTGISCYTCIAC	700	50	[9]
Type II PKS	540F	Partial KS genes of Type II PKS	GGITGCACSTCIGGIM TSGAC		68	[23]
	1100R	Partial KS genes of Type II PKS	CCGATSGCICCSAGIGAGTG		68	[23]
	KSα	PKS-II, KSα and KSβ domains/designed to target conserved sequences	TSGRCTACRTCAACGGSCACGG	600–700	58	[24]
	KSβ		TACSAGTCSWTCGCCTGGTTC	600–700	58	[24]
NRPS	A3F	NRPS adenylation domains/alignments of ketosynthase, acyltransferase and adenylation sequences	GCSTACSYSATSTACACSTCSGG		65	[24]
	A7R		SASGTCVCCSGTSCGGTAS		65	[24]
	MTF2	Adenylation A domain of NRPS	GCNGGYGGYGCNTAYGTNCC	1,000	60	[24]
	MTR	Adenylation A domain of NRPS	CCNCGDATYTTNACYTG	1,000	60	[24]

*Annealing temperature was modified based on the results of preliminary experiments for this study.

J. Microbiol. Biotechnol. 2019;29:1144~1154 https://doi.org/10.4014/jmb.1906.06037