Fig. 5.

Fig. 5. Synchronized lysis circuit of S. Typhimurium releases the anti-cancer drug hemolysin E. (A) AHL molecules that were produced by the luxI gene on an activator plasmid bind to LuxR synthesized by S. Typhimurium. LuxR-AHL complex binds to and transcriptionally activates the luxI promoter, which auto-synthesizes AHL and triggers the expression of φX174E, hlyE, and sfGFP. (B) AHL diffuses to nearby cells. (C) When AHL concentration reaches a quorum threshold in the bacterial population, cell lysis occurs for the release of hemolysin E. (D) A small number of surviving bacteria continue to grow and re-start the process in a cyclical manner.

© J. Microbiol. Biotechnol.