JMB

eISSN 1738-8872
pISSN 1017-7825

Table. 3.

Table. 3.

Different strategy for BGC cloning.

BGC cloning steps Strategies Representative examples Ref
Acquisition of the target BGC from the native host genome Genomic library Cosmid, fosmid, BAC, and PAC [135]
Cut off both ends of target BGC Restriction: pSBAC [113]
Integrase: IR [114]
CRISPR: CATCH, mCRISTAR, and CRISPR-TAR [96, 97, 120]
PCR amplification DNA assembler [116]
Ligation or assembly of the target BGC to the vector In vitro Sticky end ligation: pSBAC [113]
Blunt end ligation: ICE [95]
Gibson assembly: CATCH and MSGE [89, 96]
In vivo Recombination in native host: IR [114]
Recombination in E. coli: LLHR [117]
Recombination in yeast: TAR, DNA assembler, DiPac, and mCRISTAR [97, 115, 116, 118]
Transferring BGC vector to the expression host Conjugation pUWLcre [136]
Protoplast transformation pSKC2 and pOJ446 [137]
Target secondary metabolite production by expression of the BGC vector Integrative pSET152, pCAP01, and pESAC [118, 138]
Replicative pSKC2 and pUWL201 [139]
J. Microbiol. Biotechnol. 2019;29:667~686 https://doi.org/10.4014/jmb.1904.04015
© J. Microbiol. Biotechnol.