Fig. 2. Detection of recombinant GnRH proteins by western blotting. Recombinant GnRH-I, GnRH-II, and GnRH-I+II peptides conjugated to STF-2, were detected using anti-GnRH and anti-flagellin polyclonal antibodies. The size of the recombinant GnRH proteins was calculated at approximately 72 kDa.