Fig. 4.

Fig. 4. PTPRB controls brown adipogenic differentiation by modulation of VEGFR2 phosphorylation. (A) The changes in the phosphorylation level of VEGFR2 during brown adipogenesis were monitored by western blot analysis. Cells overexpressing wild-type PTPRB or catalytic inactive mutant PTPRB were serum-starved for 12 h and treated with a differentiation-inducing agent for the indicated times. (B) The phosphorylation level of VEGFR2 was confirmed during brown adipocyte differentiation in the absence or presence of CP547632 (pharmacological inhibitor of VEGFR2 phosphorylation). (C) The expression levels of brown adipogenic genes were analyzed by real-time PCR. PTPRB or PTPR-CS expressing brown preadipocytes in the absence or presence of CP547632 were induced to undergo brown adipogenic differentiation for 6 days according to standard procedures. Total RNA was extracted on day 6 of the differentiation. The data represent the means ± SD (n = 3, *p < 0.05).

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