Fig. 2.

Fig. 2. The overexpression of PTPRB suppresses brown adipocyte differentiation of the immortalized brown preadipocytes. Immortalized brown preadipocytes were stably infected with retroviruses (pRetroX-IRES-ZsGreen1) expressing Control Vector, FLAG-tagged PTPRB or PTPRB-CS (catalytically inactive; catalytic Cys 1907 was replaced with Ser). Infected cells were selected by FACS sorting. (A) GFP expression was monitored directly using fluorescence microscopy. (B) The expression level of PTPRB was confirmed by Real-time PCR and western blot analysis. (C) The cells expressing wild-type or mutant PTPRB were induced to differentiate into brown adipocytes through the brown adipogenic program for 6 days. Then, cells were stained with Oil-Red-O to visualize the lipid droplets. (D) The mRNA expression levels of UCP- 1, PGC-1α, PRDM16, PPAR-γ, and CIDEA were analyzed by Real-time PCR. The data represent the means ± SD (n = 3, *p < 0.05).

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