JMB

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Table. 1.

Table. 1.

Strains and plasmids used in this study.

Strains Genotypes
S. cerevisiae BY4742 MATα leu2△0 ura3△0 his3-△1 lys2-△0
S. cerevisiae 2805 MATα pep4::HIS4 prb1-1.6R can1 GAL2 his3-200 ura3-52
S. cerevisiae FY834 MATα ura3-52 his3-△200 leu2-△1 lys2-△202 trp1-△63
Plasmids Descriptions
pGEX-AgaH71 β-agarase from Pseudoalteromonas hodoensis H7
pGEX-AgaG1 β-agarase from Alteromomas sp. GNUM-1
pET-NABH558 Neoagarobiose hydrolase from Gayadomonas joobiniege G7
pGMFα- XYLP Gal10p-MFα signal sequence-Gal7t, Ura3 selective marker
pGMFα-AgaG1 Gal10p-MFαs.s-AGAG1-Gal7t, Ura3 selective marker
pGMFα-AgaH71 Gal10p-MFαs.s-AGAH71-Gal7t, Ura3 selective marker
pGMFα-NABH Gal10p-MFαs.s-NABH558-Gal7t, Ura3 selective marker
pGMFα-HGN AGAH71 EC-AGAG1 EC-NABH EC, Ura3 selective marker
J. Microbiol. Biotechnol. 2019;29:625~632 https://doi.org/10.4014/jmb.1902.02012
© J. Microbiol. Biotechnol.