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Table. 4.

Table. 4.

Enzyme kinetics of wild-type PhyE and the thermostable mutant P56214.

Variant Vmax a K M (mM) a K cat (s-1) K cat/K M
WT PhyE 1.51±0.03 1.56±0.13 768.68±4.32 439.68
P56214 1.30±0.12 2.58±0.02 578.91±8.12 224.38

*
p<0.05, Student’s t-test; calculated using GraphPad Prism 6.

J. Microbiol. Biotechnol. 2019;29:419~428 https://doi.org/10.4014/jmb.1811.11017
© J. Microbiol. Biotechnol.