Fig. 4.
A, the iterative order of the different mutant libraries. WT and P15-P562143 represent the wild-type phytase and the different iterative mutant libraries, respectively. The details of the strains’ genetics and phenotypes are listed in
Table 1. Only the positive iterative roadmap towards enhanced heat resistance of engineered phytase was shown in the graph. B, SDS-PAGE and western blot of the engineered phytase mutant P56214. The arrows represent the phytase band.
© J. Microbiol. Biotechnol.