Fig. 4.

Fig. 4. Effect of silibinin on the activity of Wnt/ β-catenin signaling on 3D DP spheroids. (A) The effect of silibinin on the luciferase activity of TCF/LEF reporter plasmid in 3D DP spheroids. DP cells were transfected with the reporter and β-galactosidase plasmids. Cells were then grown in a 3D-cultured system followed by treatment with silibinin for 24 h. The luciferase activity was determined by normalizing the β-galactosidase activity. (B and C) The effect of silibinin on the expression levels of target genes of Wnt/β- catenin signaling, WNT5A (B) and LEF1 (C) in 3D DP spheroids. DP cells were grown in a 3D-cultured system, treated with the indicated concentrations of silibinin (0 or 10 μM) for 24 h, and then the mRNA levels of these genes were assessed by qRT-PCR analysis. The data are presented as the mean ± S.D. of three independent experiments. Values of p < 0.05 were considered statistically significant.

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