Fig. 2.

Fig. 2. Effect of AKT activation on the viability in silibinin-treated 3D DP cells. (A) The effect of silibinin on AKT phosphorylation/activation in 3D-cultured DP cells. DP cells were grown in a 3D-cultured system, treated with different concentrations of silibinin (1–10 μM) for 24 h, and cell viability was determined through immunoblotting assay with specific antibodies. Quantification of p-Akt protein was carried out using the Image-J program and normalized to total Akt protein levels. (B) AKT-dependent regulation of cell viability in silibinin-treated 3D DP cells. DP cells were grown in a 3D-cultured system and co-treated with different concentrations of silibinin and LY294002 for 24 h. Cell viability was determined through WST-1 assay. The results are expressed as percent cell viability relative to the control. The data are presented as the mean ± S.D. of three independent experiments. Values indicated by * were considered statistically significant with p < 0.05.

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