Fig. 2.

Fig. 2. Effect of ECE on bone resorption of RANKL-induced RAW 264.7 macrophages. Cells were incubated for 6 days on fluoresceinamine-labeled chondroitin sulfate-labeled calcium phosphate-coated plates in the presence of RANKL (50 ng/ml), M-CSF (25 ng/ml), and samples. Bone absorption activity was evaluated by measuring the fluorescence intensity produced by the decomposition of calcium phosphate with fluorescence. Bars with different letters indicate significant differences at p <0.05. inhibited RANKL-induced bone resorptive pit formation. Based on these results, ECE treatment actively suppresses osteoclast differentiation and bone resorption.

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