Fig. 1.

Fig. 1. Effects of ECE on TRAP formation and activity in RANKL-induced RAW 264.7 macrophages. ECE: Ecklonia cava extract, RANKL: receptor activator of nuclear factor kappa-B ligand. (A) TRAP staining image. Cells were incubated for 10 days in the presence of RANKL (50 ng/ml), M-CSF (25 ng/ml), and sample (ECE 100 μg/ml). TRAP staining was observed using a microscope and TRAP (+) stained multinucleated cells containing ≥ 3 nuclei were considered osteoclasts. Scale bar unit: 10 μm. (B) TRAP activity. Activity was determined after 4 days of RANKL treatment. (C) Cytotoxicity. Cell viability was determined at 10 days using the MTT assay. Bars with different letters indicate significant differences at p <0.05.

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