Fig. 5.

Fig. 5. Effect of ammonium nitrate on Xoo biofilm formation (A) and cell growth (B). Cells were incubated in 96-well polyvinyl chloride microplates for 24 h with XOM2 medium in which L-methionine and sodium L-(+)- glutamate monohydrate were replaced with ammonium nitrate. Biofilms were quantified using crystal violet (CV in the y-axis label; ■). Cell growth was measured independently by culturing cells in test tubes. The number of colony-forming units (CFU) was calculated using a conversion factor of 1.12 × 10⁹ CFU/Abs₆₀₀ (▲). Data represent mean ± standard deviation from 10 independent experiments for biofilm and from 2 independent experiments for cell density. The mean values showing a statistically significant difference in comparison with the control value at 0 mM were denoted by * (p < 0.05).

© J. Microbiol. Biotechnol.