Fig. 4.
Effect of amino acids on Xoo biofilm formation. Cells were incubated in 96-well polyvinyl chloride microplates for 24 h with XOM2 medium in which L-methionine and sodium L-(+)- glutamate monohydrate were replaced with the indicated amino acid. Amino acids at 50 mM (A) and 100 mM (B) were tested. Biofilms were quantified using crystal violet (CV in the y-axis label). Data represent mean ± standard deviation from at least 5 independent experiments, except for proline, which was used in 3 independent experiments. The mean values showing a statistically significant difference in comparison with the control value without amino acid were denoted by * (p < 0.05).