eISSN 1738-8872
pISSN 1017-7825
pISSN 1017-7825
Table. 1.
Table. 1.
Oligonucleotide primers used for the qRT-PCR analysis of adhesion-related gene expression.
| Gene | Sequence of PCR primers (5’ to 3’) | Target locus | Product | Reference |
|---|---|---|---|---|
| Forward : GCTGATGTTACTGCTGATGC Reverse : ATCCGTATTTTGAACGGGTA |
LRHM_2281 | putative cell surface protein | PMID 19820099 | |
| Forward : CAACTTGATGGGACAACGTA Reverse : TCTGGTGCTTTTGTTTCTGA |
LRHM_0428 | Cell surface protein | PMID 19820099 | |
| Forward : GATTATCGGGTTGATTCTGG Reverse : AAATCGCCTTCGTACATCTC |
LGG_02339 | Pili_PIN/TRAM domain-containing protein | PMID 19820099 | |
| Forward : CAACTTGATGGGACAACGTA Reverse : TTTGCAGGATTGCTTTGATA |
LGG_00444 | Pilus protein | PMID 19820099 | |
| Forward : CTAAATCCTTCCGTCCGTTA Reverse : CTCAACGTCGTTTGTGCTAC |
LGG_00443 | Pilus protein | PMID 19820099 | |
| Sequence of PCR primers (5’ to 3’) | Target locus | Product | Reference | |
| Forward : GATGGTTCTCGGGTTTAATG Reverse : ACCCACGTCAATCGATAGTT |
LGG_00442 | pilus protein | PMID 19820099 | |
| Forward : AACTATCCATTCGGGTTTGA Reverse : GTTGTCGGATCAAGGATTTC |
LGG_02370 | Pilus protein | PMID 19820099 | |
| Forward : TGAAGTTGGAAAAGCGTACA Reverse : AGGAAAAGTTCCTGATGCTG |
AB968049 | Mucus binding factor | PMID 19820099 | |
| Forward : GATCGTTTCTGCAGGTTCTT Reverse : CCGTTCAATTCTGGGATAAC |
LGG_00933 | Type I glyceraldehyde-3-phosphate dehydrogenase | PMID 19820099 |
J. Microbiol. Biotechnol. 2018;28:1604~1613 https://doi.org/10.4014/jmb.1807.07033
© J. Microbiol. Biotechnol.