Fig. 5.
The effects of octaminomycins A (1) and B (2) on the protein expression and proteolytic activities of MMPs. Serum-starved HUVECs were treated with 1 and 2 in the presence or absense of VEGF (30 ng/ml) for 24 h. (A) Protein levels were determined by Western blotting using specific antibodies and further quantified by densitometry. The level of β-actin was used as an internal control. (B) Enzymatic activities of MMPs were detected by gelatin zymogram analysis and further quantified by densitometry.