Fig. 2.
The in vitro anti-angiogenic activities of octaminomycins A (1) and B (2). Serum-starved HUVECs were stimulated with VEGF (30 ng/ml) in the presence or absense of 1 and 2. (A) The effects of 1 and 2 on the proliferation of HUVECs. The growth of HUVECs was determined after 72 h using an MTT colorimetric assay. (B) The effects of 1 and 2 on the migration of HUVECs. The closure of the wound was visualized after 24 h under a microscope. (C) The effects of 1 and 2 on the adhesion of HUVECs. The attached cells to laminin after 2 h were counted under a microscope. (D) The effects of 1 and 2 on the invasion of HUVECs. The invaded cells after 24 h were stained with hematoxylin/eosin and visualized under a microscope. (E) The effects of 1 and 2 on the tube-forming ability of HUVECs. The formed tubes after 6 h were visualized under a microscope. *p < 0.05 versus the VEGF control.