Fig. 3.

Fig. 3. Conessine treatment suppresses the expression of MuRF1 and atrogin-1. (A) Conessine decreased the mRNA levels of MuRF1 and atrogin-1. C2C12 myotubes were either mock-treated or treated with dexamethasone (Dex, 100 μM), and then treated with conessine (10 μM). MuRF1 and atrogin-1 mRNA levels were analyzed via quantitative RT-PCR. Dexamethasone treatment vs. dexamethasone and conessine treatment, *p < 0.001. (B) Conessine decreased the level of MuRF1 and atrogin-1 protein. The cells were collected and subjected to western blot assay using anti-MuRF1 antibody and anti-atrogin-1 antibody. (C) Densitometric quantification of the MuRF1 and atrogin-1 protein expression shown in (B). Dexamethasone treatment vs. dexamethasone and conessine treatment, *p < 0.05, *p < 0.01.

© J. Microbiol. Biotechnol.