Fig. 2.

Fig. 2. Conessine treatment suppresses p53-, NF-κB-, and FoxO₃a-dependent transcription. (A) Conessine treatment suppressed p53-dependent transcription. HEK293 cells were transfected with WWP-Luc and the plasmid encoding p53. After 24 h, the cells were treated with the indicated concentrations (0, 2.5, 5, 10, and 20 μM) of conessine for 24 h and luciferase activity was measured. The experiment was performed in triplicate, and the graphs show the average and standard error. Control vs. conessine treatment, *p < 0.05, **p < 0.005. (B) Conessine treatment suppressed NF-κB-dependent transcription. HEK293 cells were co-transfected with pELAM-Luc. Cells were treated with conessine and TNFα (1 ng/ml). Control vs. conessine treatment, *p < 0.05. (C) Conessine treatment suppressed FoxO₃adependent transcription. HEK293 cells were transfected with FHRE-Luc and then treated with conessine. Control vs. conessine treatment, *p < 0.001, **p < 0.0001.

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