Fig. 1.

Fig. 1. Signaling pathway screening of conessine. (A) Chemical structure of conessine. (B-I) Luciferase reporter assay with conessine. HEK293 cells were transfected with various luciferase constructs (hTERT-Luc, AP1-Luc, ISRE-Luc, 3TP-Luc, pOT-Luc, WWP-Luc, pELAM-Luc, and FHRE-Luc) and internal control plasmids (pCMVRL). Twenty-four hours after transfection, the cells were treated with conessine (20 μM) for 24 h and luciferase reporter activity was measured. Luciferase activity was normalized with that of Renilla luciferase. The experiment was performed at least in triplicate, and the graphs show the average and standard error. Mock vs. conessine treatment, *p < 0.05, **p < 0.005; NS, not significant.

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