Fig. 3.

Fig. 3. Expression of the recombinant protease from Janthinobacterium lividum PAMC 26541 in E. coli. (A) SDS-PAGE analysis (12% polyacrylamide gel) of recombinant E. coli cells. M, Standard protein marker; 1, cell pellet containing pET-28a(+); 2, cell pellet containing pET28a-pro before induction; 3, cell pellet containing pET28a-pro after induction. (B) SDS-PAGE analysis (8% polyacrylamide gel) of cell lysate. M, Standard protein marker; 4, supernatant of cell lysate (soluble proteins) containing pET-28a(+); 5, supernatant of cell lysate containing pET28a-pro before induction; 6, supernatant of cell lysate containing pET28a-pro after induction.

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