eISSN 1738-8872
pISSN 1017-7825
pISSN 1017-7825
Table. 1.
Table. 1.
Effect of various metal ions and chemical reagents on the activity of the purified protease from
| Reagent | Concentration | Relative enzyme activity (%) |
|---|---|---|
| (Control) | None | 100 ± 4 |
| K2SO4 | 10 mM | 118 ± 7 |
| Na2SO4 | 10 mM | 115 ± 3 |
| MgSO4 | 10 mM | 87 ± 3 |
| CaCl2 | 10 mM | 18 ± 6 |
| CoCl2 | 10 mM | 11 ± 2 |
| MnSO4 | 10 mM | 7 ± 10 |
| FeSO4 | 10 mM | 6 ± 8 |
| CuSO4 | 10 mM | 4 ± 6 |
| ZnSO4 | 10 mM | 2 ± 5 |
| Tween-20 | 1 mM | 99 ± 3 |
| EDTA | 1 mM | 91 ± 5 |
| DTT | 1 mM | 90 ± 4 |
| β-Mercaptoethanol | 1 mM | 85 ± 7 |
| PMSF | 1 mM | 45 ± 5 |
| Ethanol | 3% | 96 ± 3 |
J. Microbiol. Biotechnol. 2018;28:448~453 https://doi.org/10.4014/jmb.1711.11006
© J. Microbiol. Biotechnol.