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Fig. 1.

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Fig. 1. Tris-Tricine PAGE analysis of recombinant protein expression and production. (A-C) 12% Tris-Tricine PAGE analysis that confirmed the expression levels of expression host cells containing a single insert of YK5, YK8, and YK11, respectively. Lane 1: molecular weight marker; Lanes 2-4: BL21(DE3)pLysS; Lanes 5-7: C41(DE3); Lanes 8-10: C43(DE3). (D-F) 12% Tris- Tricine PAGE showing the expression and purification steps of analog peptides. Lane 1: molecular weight marker; Lane 2: before induction, Lane 3: after induction; Lane 4: supernatant of cell lysis; Lane 5: pellet of cell lysis; Lane 6: after Ni-NTA affinity chromatography; Lane 7: after CNBr cleavage.
J. Microbiol. Biotechnol. 2018;28:381~390 https://doi.org/10.4014/jmb.1711.11057
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