Fig. 3.
BPE-induced stimulation of osteoblast differentiation in alveolar bone of aged rats. (A) The mRNA levels of ALP, COL1A1, OPG, and RANKL in alveolar bone were estimated using RT-PCR; β-actin was used as the housekeeping gene. (B) The protein expression was examined by western blotting. All data are expressed as the mean ± SD (% of control) of eight rats per group. ##p < 0.01 (young control vs. aged control group) and **p < 0.01 (aged control vs. BPE-treated aged group).