Fig. 2.
Effect of BPE on histological changes in age-related periodontitis in rats. (A) Histological analysis of periodontium using hematoxylin and eosin staining (×200 magnification). Black arrow, cell infiltration; yellow arrow, alveolar bone resorption; A, alveolar bone; PDL, periodontal ligament; C, cementum; D, dentin. (B) Histological analysis of periodontium using immunohistochemical staining (×400 magnification). Yellow arrow, expressed proteins; A, alveolar bone; PDL, periodontal ligament. (C) Quantification of the relative stained area in the immunohistochemical images. The relative stained areas are expressed as the mean ± SD (% of control) of three rats per group. ##p < 0.01 (young control vs. aged control group) and **p < 0.01 (aged control vs. BPE-treated aged group).