2013 ; 23(9):
|Author||Tonči Rezić, Damir Oros, Iva Marković, Daniel Kracher, Roland Ludwig, Božidar Šantek|
|Affiliation||Faculty of Food Technology and Biotechnology, University of Zagreb University, 10000 Zagreb, Pierottijeva 6, Croatia|
|Title||Integrated Hydrolyzation and Fermentation of Sugar Beet Pulp to Bioethanol|
J. Microbiol. Biotechnol.2013 ; 23(9):
|Abstract||Sugar beet pulp is an abundant industrial waste material that holds a great potential for
bioethanol production owing to its high content of cellulose, hemicelluloses, and pectin. Its
structural and chemical robustness limits the yield of fermentable sugars obtained by
hydrolyzation and represents the main bottleneck for bioethanol production. Physical
(ultrasound and thermal) pretreatment methods were tested and combined with enzymatic
hydrolysis by cellulase and pectinase to evaluate the most efficient strategy. The optimized
hydrolysis process was combined with a fermentation step using a Saccharomyces cerevisiae
strain for ethanol production in a single-tank bioreactor. Optimal sugar beet pulp conversion
was achieved at a concentration of 60 g/l (39% of dry weight) and a bioreactor stirrer speed of
960 rpm. The maximum ethanol yield was 0.1 g ethanol/g of dry weight (0.25 g ethanol/g
total sugar content), the efficiency of ethanol production was 49%, and the productivity of the
bioprocess was 0.29 g/l·h, respectively.|
|Keywords||Sugar beet pulp, bioethanol, ultrasound pretreatment, thermal pretreatment, enzymatic hydrolysis, Saccharomyces cerevisiae|
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