2013 ; 23(4):
|Author||Sarawut Koksunan, Sukanda Vichitphan, Lakkana Laopaiboon, Kanit Vichitphan, Jaehong Han|
|Affiliation||Department of Biotechnology, Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen 40002, Thailand|
|Title||Growth and Cyanide Degradation of Azotobacter vinelandii in Cyanide-Containing Wastewater System|
J. Microbiol. Biotechnol.2013 ; 23(4):
|Abstract||Azotobacter vinelandii, a strict aerobic nitrogen-fixing
bacterium, has been extensively studied with regard to the
ability of N2-fixation due to its high expression of
nitrogenase and fast growth. Because nitrogenase can also
reduce cyanide to ammonia and methane, cyanide
degradation by A. vinelandii has been studied for the
application in the bioremediation of cyanide-contaminated
wastewater. Cyanide degradation by A. vinelandii in NFS
(nitrogen-free sucrose) medium was examined in terms of
cell growth and cyanide reduction, and the results were
applied for cyanide-contaminated cassava mill wastewater.
From the NFS medium study in the 300 ml flask, it was
found that A. vinelandii in the early stationary growth
phase could reduce cyanide more rapidly than the cells in
the exponential growth phase, and 84.4% of cyanide was
degraded in 66 h incubation upon addition of 3.0 mM of
NaCN. The resting cells of A. vinelandii could also reduce
cyanide concentration by 90.4% with 3.0 mM of NaCN in
the large-scale (3 L) fermentation with the same incubation
time. Finally, the optimized conditions were applied to the
cassava mill wastewater bioremediation, and A. vinelandii
was able to reduce the cyanide concentration by 69.7%
after 66 h in the cassava mill wastewater containing
4.0 mM of NaCN in the 3 L fermenter. Related to cyanide
degradation in the cassava mill wastewater, nitrogenase
was the responsible enzyme, which was confirmed by
methane production. These findings would be helpful to
design a practical bioremediation system for the treatment
of cyanide-contaminated wastewater.|
|Keywords||Azotobacter vinelandii, Bioremediation, Cyanide, Nitrogenase, Wastewater|
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