Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
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2013 ; 23(3): 357~363

AuthorPiotr Jarocki, Marcin Podlesny, Jaroslaw Pawelec, Agata Malinowska, Sylwia Kowalczyk, Zdzislaw Targonski
AffiliationDepartment of Biotechnology, Human Nutrition and Food Commodities, University of Life Sciences in Lublin, 8 Skromna St., 20-704 Lublin, Poland
TitleSpontaneous Release of Bacteriophage Particles by Lactobacillus rhamnosus Pen
PublicationInfo J. Microbiol. Biotechnol.2013 ; 23(3): 357~363
AbstractThe identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein. Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.
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Keywordslysogeny, bacteriophage, Lactobacillus rhamnosus, major tail protein
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