Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
Condition  Expression
When you enter More than two words, please use ‘and , or’ operation by means of putting ‘,(Comma Mark)’ between each word.

2013 ; 23(10): 1372~1380

AuthorSanjog Garyali, Anil Kumar, M. Sudhakara Reddy
AffiliationDepartment of Biotechnology, Thapar University, Patiala 147004, Punjab, India
TitleTaxol Production by an Endophytic Fungus, Fusarium redolens, Isolated from Himalayan Yew
PublicationInfo J. Microbiol. Biotechnol.2013 ; 23(10): 1372~1380
AbstractDifferent endophytic fungi isolated from Himalayan Yew plants were tested for their ability to produce taxol. The BAPT gene (C-13 phenylpropanoid side chain-CoA acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxolproducing endophytic fungi. Taxol extracted from fungal strain TBPJ-B was identified by HPLC and MS analysis. Strain TBPJ-B was identified as Fusarium redolens based on the morphology and internal transcribed spacer region of nrDNA analysis. HPLC quantification of fungal taxol showed that F. redolens was capable of producing 66 μg/l of taxol in fermentation broth. The antitumour activity of the fungal taxol was tested by potato disc tumor induction assay using Agrobacterium tumefaciens as the tumor induction agent. The present study results showed that PCR amplification of genes involved in taxol biosynthesis is an efficient and reliable method for prescreening taxol-producing fungi. We are reporting for the first time the production of taxol by F. redolens from Taxus baccata L. subsp. wallichiana (Zucc.) Pilger. This study offers important information and a new source for the production of the important anticancer drug taxol by endophytic fungus fermentation.
Full-Text(PDF)
KeywordsAgrobacterium tumefaciens, endophytic fungi, ITS, Fusarium redolens, Himalayan yew, Taxus baccata
References
  1. Barnett HL, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi. 4th Ed. APS Press, St. Paul, Minnesota.
  2. Cardellina JH. 1991. HPLC separation of taxol and cephalomannine. J. Liquid Chromatogr. 14: 659-665.
    CrossRef
  3. Coker PS, Radecke J, Guy C, Camper ND. 2003. Potato disc tumour induction assay: a multiple mode of drug action assay. J. Phytomedicine 10: 133-138.
    Pubmed CrossRef
  4. Dai W, Tao W. 2008. Preliminarly study on fermentation conditions of taxol-producing endophytic fungus. Chem. Ind. Eng. Prog. 27: 883-886.
  5. Deng B W, Liu K H, Chen WQ, Ding XW, Xie XC. 2 009. Fusarium solani, Tax-3, a new endophytic taxol-producing fungus from Taxus chinensis. World J. Microbiol. Biotechnol. 25: 139-143.
    CrossRef
  6. Gangadevi V, Muthumary J. 2008. Taxol, an anticancer drug produced by an endophytic fungus Bartalinia robillardoides, Tassi, isolated from a medicinal plant, Aegle marmelos Correa ex Roxb. World J. Microbiol. Biotechnol. 24: 717-724.
    CrossRef
  7. Ganley RJ, Brunsfeld SJ, Newcombe G. 2004. A community of unknown, endophytic fungi in western white pine. Proc. Natl. Acad. Sci. USA 101: 10107-10112.
    Pubmed CrossRef
  8. Huang Y, Wang J, Li G, Zheng Z, Su W. 2001. Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei, Cephalataxus fortunei and Torreyagrandis. FEMS Immunol. Med. Microbiol. 31: 163-167.
    Pubmed CrossRef
  9. Kumar S, Tamura K, Peterson D, Peterson N, Stecher G, Nei M. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 27312739.
  10. Li CT, Li Y, Wang QJ, Sung CK. 2008. Taxol production by Fusarium arthrosporioides isolated from yew, Taxus cuspidata. J. Med. Biochem. 27: 454-458.
    CrossRef
  11. Li J, Hu Y, Chen W, Lin Z. 2006. Identification and pilot study of Taxus endophytic fungi’s taxol-producing correlation gene BAPT. Biotech. Bull. S1: 356-371.
  12. Lin X, Lu CH, Huang YJ, Zheng ZH, Su WJ, Shen YM. 2007. Endophytic fungi from a pharmaceutical plant, Camptotheca acuminata: isolation, identification and bioactivity. World J. Microbiol. Biotechnol. 23: 1037-1040.
    CrossRef
  13. Liu K, Ding X, Deng B, Chen W. 2009. Isolation and characterization of endophytic taxol-producing fungi from Taxus chinensis. J. Ind. Microbiol. Biotechnol. 36: 1171-1177.
    Pubmed CrossRef
  14. Mc Laughlin JL, Rogers LL. 1998. The use of biological assays to evaluate botanicals. Drug Inform. J. 32: 513-524.
  15. Miller JD, Mackenzie S, Foto M, Adams GW, Findlay JA. 2002. Needles of white spruce inoculated with rugulosinproducing endophytes contain ruglosin reducing spruce budworm growth rate. Mycol. Res. 106: 471-479.
    CrossRef
  16. Nadeem M , Rikhari H C, Kumar A , Palni LMS, Nandi SK. 2002. Taxol content in the bark of Himalayan Yew in relation to tree age and sex. Phytochemistry 60: 627-631.
    CrossRef
  17. Pandey AK, Reddy MS, Suryanarayanan TS. 2003. ITS-RFLP and ITS sequence analysis of a foliar endophytic Phyllosticta from different tropical trees. Mycol. Res. 107: 439-444.
    Pubmed CrossRef
  18. Porras-Alfaro A, Herrera J, Sinsabaugh RL, Odenbach KJ, Lowrey T, Natvig DO. 2008. Novel root fungal consortium associated with a dominant desert grass. Appl. Environ. Microbiol. 74: 2805-2813.
    Pubmed CrossRef
  19. Radman RS, Sheehan K B, Stout RG, Rodriquez R J, Henson JM. 2002. Thermotolerance generated by plant/fungal symbiosis. Science 298: 1581.
    CrossRef
  20. Stierle A, Strobel GA, Stierle D. 1993. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 260: 214-216.
    Pubmed CrossRef
  21. Strobel G A, Hess WM, Ford E, Sidhu R S, Yang X. 1 996. Taxol from fungal endophytes and issue of biodiversity. J. Ind. Microbiol. 17: 417-423.
  22. Strobel G, Yang X, Sears J , Kramer R, Sidhu RS, Hess WM. 1996. Taxol from Pestalopsis microspora, an endophytic fungus from Taxus wallachiana. Microbiology 142: 435-440.
    Pubmed CrossRef
  23. Tan RX, Zou WX. 2001. Endophytes: a rich source of functional metabolites. Nat. Prod. Rep. 18: 448-459.
    Pubmed CrossRef
  24. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The Clustal X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24: 4876-4882.
    CrossRef
  25. Walker K, Croteau R. 2000. Molecular cloning of 10deacetylbaccatin III-10-O-acetly transferase cDNA from Taxus and functional expression in Escherichia coli. Proc. Natl. Acad. Sci. USA 97: 583-587.
    Pubmed CrossRef
  26. Walker K, Fujisaki S, Long R, Croteau R. 2002. Molecular cloning and heterologous expression of the C-13 phenyl propanoid side chain-CoA acyltransferase that functions in taxol biosynthesis. Proc. Natl. Acad. Sci. USA 99: 1271512720.
    Pubmed CrossRef
  27. Wang J, Li G, Lu H, Zheng Z , Huang Y, Su W. 2000. Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei. FEMS Microbiol. Lett. 193: 249-253.
    Pubmed CrossRef
  28. Wei JC. 1979. Handbook for Identification of Fungi, pp. 405-649. Shanghai Science and Technology Press, Shanghai.
  29. Wheeler N C, Jech K, Masters S , Brobst SW, Alvarado AB, Hoover AJ, et al. 1992. Effects of genetic, epigenetic, and environmental factors on taxol content in Taxus brevifolia and related species. J. Nat. Prod. 55: 432-440.
    Pubmed CrossRef
  30. White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In Innis M A, Gelfand D H, Sninsky JJ, White TJ (eds.). PCR Protocols: A Guide to Methods and Application. Academic Press Inc., San Diego.
    Pubmed
  31. Zhang CL, Liu SP, Lin FC, Kubicek CP, Druzhinina IS. 2007. Trichoderma taxi sp. nov., an endophytic fungus from Chinese yew Taxus mairei. FEMS Microbiol. Lett. 207: 90-96.
    Pubmed CrossRef
  32. Zhang D, Yang Y, Castlebury L A, Cerniglia C E. 1 996. A method for the large scale isolation of high transformation efficiency fungal genomic DNA. FEMS Microbiol. Lett. 145:261-265.
    Pubmed CrossRef
  33. Zhang P, Zhou P, Chen J, Yu H, Yu L. 2008. Screening of taxol-producing fungi based on PCR amplification from Taxus. Biotechnol. Lett. 30: 2119-2123.
    Pubmed CrossRef
  34. Zhao J, Zhou L, Wang J, Shan T, Zhong L, Liu X, et al. 2010. Endophytic fungi for producing bioactive compounds originally from their host plants. Curr. Res. Technol. Education Topics Appl. Microbiol. Microb. Biotechnol. 567-576.
  35. Zhao K, Ping W, Li Q, Hao S, Zhao L, Gao T , et al. 2009. Aspergillus niger var. taxi, a new species variant of taxolproducing fungus isolated from Taxus cuspidate in China. J. Appl. Microbiol. 107: 1202-1207.
    Pubmed CrossRef
  36. Zhao K, Zhao LF, Jin Y, Wei HX, Ping WX, Zhou DP. 2008. Isolation of a taxol-producing endophytic fungus and inhibiting effect of the fungus metabolites on HeLa cell. Mycosystema 5: 210-217.
  37. Zhou X, Wang Z, Jiang K, Wei Y , Lin J, Sun X , et al. 2007. Screening of taxol-producing endophytic fungi from Taxus chinensis var. mairei. Appl. Biochem. Microbiol. 43: 439-443.
    CrossRef
Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to jmb@jmb.or.kr
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by INFOrang.co., Ltd