Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
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2013 ; 23(1): 76~84

AuthorYeun Hong, Hee-Seok Yang, Hae-Choon Chang, Hae-Yeong Kim
AffiliationInstitute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 446-701, Korea
TitleComparison of Bacterial Community Changes in Fermenting Kimchi at Two Different Temperatures Using a Denaturing Gradient Gel Electrophoresis Analysis
PublicationInfo J. Microbiol. Biotechnol.2013 ; 23(1): 76~84
AbstractA polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique followed by sequencing of the 16S rDNA fragments eluted from the bands of interest on denaturing gradient gels was used to monitor changes in the bacterial microflora of two commercial kimchi, salted cabbage, and ingredient mix samples during 30 days of fermentation at 4oC and 10oC. Leuconostoc (Lc.) was the dominant lactic acid bacteria (LAB) over Lactobacillus (Lb.) species at 4oC. Weissella confusa was detected in the ingredient mix and also in kimchi samples throughout fermentation in both samples at 4oC and 10oC. Lc. gelidum was detected as the dominant LAB at 4oC in both samples. The temperature affected the LAB profile of kimchi by varing the pH, which was primarily caused by the temperature-dependent competition among different LAB species in kimchi. At 4oC, the sample variations in pH and titratable acidity were more conspicuous owing to the delayed growth of LAB. Temperature affected only initial decreases in pH and initial increases in viable cell counts, but affected both the initial increases and final values of titratable acidity. The initial microflora in the kimchi sample was probably determined by the microflora of the ingredient mix, not by that of the salted cabbage. The microbial distributions in the samples used in this study resembled across the different kimchi samples and the different fermentation temperatures as the numbers of LAB increased and titratable acidity decreased.
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Keywordsbacterial community, PCR-DGGE, kimchi, lactic acid bacteria
References
  1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST; a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
    Pubmed CrossRef Pubmed Central
  2. Björkroth, K. J., P. Vandamme, and H. J. Korkeala. 1998. Identification and characterization of Leuconostoc carnosum, associated with production and spoilage of vacuum-packaged, sliced, cooked ham. Appl. Environ. Microbiol. 64: 3313-3319.
    Pubmed Pubmed Central
  3. Björkroth, K. J., R. Geisen, U. Schillinger, N. Weiss, P. De Vos, W. H. Holzapfel, et al. 2000. Characterization of Leuconostoc gasicomitatum sp. nov., associated with spoiled raw tomatomarinated broiler meat strips packaged under modified-atmosphere conditions. Appl. Environ. Microbiol. 66: 3764-3772.
    Pubmed CrossRef Pubmed Central
  4. Chang, H. W., K. H. Kim, Y. D. Nam, S. W. Roh, M. S. Kim, C. O. Jeon, and J. W. Bae. 2008. Analysis of yeast and archaeal population dynamics in kimchi using denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 126: 159-166.
    Pubmed CrossRef
  5. Cho, J. H., D. Y. Lee, C. N. Yang, J. I. Jeon, J. H. Kim, and H. U. Han. 2006. Microbial population dynamics of kimchi, a fermented cabbage product. FEMS Microbiol. Lett. 257: 262267.
    Pubmed CrossRef
  6. Chung, H. K., K. M. Yeo, and M. H. Kim. 1996. Kinetic modeling for quality prediction during kimchi fermentation. J. Food Sci. Nutr. 1: 41-45.
  7. Hamasaki, Y., M. Ayaki, H. Fuchu, M. Sugiyama, and H. Morita. 2003. Behavior of psychrotrophic lactic acid bacteria isolated from spoiling cooked meat products. Appl. Environ. Microbiol. 69: 3668-3671.
    Pubmed CrossRef Pubmed Central
  8. Jung, J. Y., S. H. Lee, H. J. Lee, H. Y. Seo, W. S. Park, and C. O. Jeon. 2012. Effects of Leuconostoc mesenteroides starter cultures on microbial communities and metabolites during kimchi fermentation. Int. J. Food Microbiol. 153: 378-387.
    Pubmed CrossRef
  9. Jung, H. J., Y. Hong, H. S. Yang, H. C. Chang, and H. Y. Kim. 2012. Distribution of lactic acid bacteria in garlic (Allium sativum) and green onion (Allium fistulosum) using SDS-PAGE whole cell protein pattern comparison and 16S rRNA gene sequence analysis. 2012. Food Sci. Biotechnol. 21 [In Press].
    CrossRef
  10. Kim, M. and J. Chun. 2005. Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis. Int. J. Food Microbiol. 103: 91-96.
    Pubmed CrossRef
  11. Kim, M. H., S. T. Shim, Y. S. Kim, and K. H. Kyung. 2002. Diversity of leuconostocs on garlic surface, an extreme environment. J. Microbiol. Biotechnol. 12: 497-502.
  12. Kim, S. Y., K. S. Yoo, J. E. Kim, J. S. Kim, J. Y. Jung, Q. Jin, H. J. Eom, and N. S. Han. 2010. Diversity analysis of lactic acid bacteria in Korean rice wines by culture-independent method using PCR-denaturing gradient gel electrophoresis. Food Sci. Biotechnol. 19: 749-755.
    CrossRef
  13. Kim, T. W., J. H. Lee, S. E. Kim, M. H. Park, H. C. Chang, and H. Y. Kim. 2009. Analysis of microbial communities in doenjang, a Korean fermented soybean paste, using nested PCR-denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 131: 265-271.
    Pubmed CrossRef
  14. Kim, T. W., J. Y. Lee, H. S. Song, J. H. Park, G. E. Ji, and H. Y. Kim. 2004. Isolation and identification of Weissella kimchii from green onion by cell protein pattern analysis. J. Microbiol. Biotechnol. 14: 105-109.
  15. Kim, T. W., J. Y. Lee, S. H. Jung, Y. M. Kim, J. S. Jo, D. K. Chung, et al. 2002. Identification and distribution of predominant lactic acid bacteria in kimchi, a Korean traditional fermented food. J. Microbiol. Biotechnol. 12: 635-642.
  16. Ku, K. H., K. O. Kang, and W. J. Kim. 1988. Some quality changes during fermentation of kimchi. Korean J. Food Sci. Technol. 20: 476-482.
  17. Lee, J. S., G. Y. Heo, J. W. Lee, Y. J. Oh, J. A. Park, Y. H. Park, et al. 2005. Analysis of kimchi microflora using denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 102: 143150.
    Pubmed CrossRef
  18. Lee, M. J. and J. H. Lee. 2009. Detection of pediococci in kimchi using pediococci selective medium. Korean J. Microbiol. Biotechnol. 37: 238-242.
  19. Lee, S. H. and S. D. Kim. 1988. Effect of starters on fermentation of kimchi. J. Korean Soc. Food Nutr. 17: 342-347.
  20. Lee, Y. H. and I. W. Yang. 1970. Studies on the packaging and preservation of kimchi. J. Korean Agric. Chem. Soc. 13: 207218.
  21. Mheen, T. I. and T. W. Kwon. 1984. Effect of temperature and salt concentration on kimchi fermentation. Korean J. Food Sci. Technol. 16: 443-450.
  22. Miller, K. M., T. J. Ming, A. D. Schulze, and R. E. Withler. 1999. Denaturing gradient gel electrophoresis (DGGE): A rapid and sensitive technique to screen nucleotide sequence variation in populations. Biotechniques 27: 1016-1018.
    Pubmed
  23. Park, J. A., G. Y. Heo, J. S. Lee, Y. J. Oh, B. Y. Kim, T. I. Mheen, et al. 2003. Change of microbial communities in kimchi fermentation at low temperature. Korean J. Microbiol. 39: 4550.
  24. Park, J. M., J. H. Shin, D. W. Lee, J. C. Song, H. J. Suh, U. J. Chang, and J. M. Kim. 2010. Identification of the lactic acid bacteria in kimchi according to initial and over-ripened fermentation using PCR and 16S rRNA gene sequence analysis. Food Sci. Biotechnol. 19: 541-546.
    CrossRef
  25. Park, W. P., K. D. Park, J. H. Kim, Y. B. Cho, and M. J. Lee. 2000. Effect of washing conditions in salted Chinese cabbage on the quality of kimchi. J. Korean Soc. Food Sci. Nutr. 29:30-34.
  26. Shaw, B. G. and C. D. Harding. 1989. Leuconostoc gelidum sp. nov. and Leuconostoc carnosum sp. nov. from chill-stored meats. Int. J. Syst. Bacteriol. 39: 217-223.
    CrossRef
  27. Shin, D. H., M. S. Kim, J. S. Han, D. K. Lim, and W. S. Park. 1996. Changes of chemical composition and microflora in commercial kimchi. Korean J. Food Sci. Technol. 28: 137-145.
  28. Sneath, P. H. A. and R. R. Sokal. 1973. Numerical Taxonomy:The Principles and Practice of Numerical Classification. W. H. Freeman, San Francisco, California, USA.
  29. So, M. H. and Y. S. Lee. 1997. Influences of cultural temperature on growth rates of lactic acid bacteria isolated from kimchi. Korean J. Food Nutr. 10: 110-116.
  30. So, M. H., Y. S. Lee, H. S. Kim, E. J. Cho, and M. J. Yea. 1996. An influence of salt concentrations on growth rates of lactic acid bacteria isolated from kimchi. Korean J. Food Nutr. 9: 341-347.
  31. Song, B. K., M. M. Clyde, R. Wickneswari, and M. N. Normah. 2000. Genetic relatedness among Lansium domesticum accessions using RAPD markers. Ann. Bot. 86: 299-307.
    CrossRef
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