2012 ; 22(6):
|Author||Yoon-E Choi, Robert A. E. Butchko, Won-Bo Shim|
|Affiliation||LED Agri-bio Fusion Technology Research Center, Chonbuk National University, Iksan-si 570-752, Korea|
|Title||Proteomic Comparison of Gibberella moniliformis in Limited-Nitrogen (Fumonisin-Inducing) and Excess-Nitrogen (Fumonisin-Repressing) Conditions|
J. Microbiol. Biotechnol.2012 ; 22(6):
|Abstract||The maize pathogen Gibberella moniliformis produces
fumonisins, a group of mycotoxins associated with several
disorders in animals and humans, including cancer. The
current focus of our research is to understand the
regulatory mechanisms involved in fumonisin biosynthesis.
In this study, we employed a proteomics approach to
identify novel genes involved in the fumonisin biosynthesis
under nitrogen stress. The combination of genome sequence,
mutant strains, EST database, microarrays, and proteomics
offers an opportunity to advance our understanding of
this process. We investigated the response of the G.
moniliformis proteome in limited nitrogen (N0, fumonisininducing)
and excess nitrogen (N+, fumonisin-repressing)
conditions by one- and two-dimensional electrophoresis.
We selected 11 differentially expressed proteins, six from
limited nitrogen conditions and five from excess nitrogen
conditions, and determined the sequences by peptide mass
fingerprinting and MS/MS spectrophotometry. Subsequently,
we identified the EST sequences corresponding to the
proteins and studied their expression profiles in different
culture conditions. Through the comparative analysis of
gene and protein expression data, we identified three
candidate genes for functional analysis and our results
provided valuable clues regarding the regulatory mechanisms
of fumonisin biosynthesis.|
|Keywords||Fusarium verticillioides, Nitrogen metabolite repression, fumonisin regulation, proteomics, gene expression|
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