2012 ; 22(6):
|Author||Jin-Su Kim, Han-Na Lee, Pil Kim, Heung-Shick Lee, Eung-Soo Kim|
|Affiliation||Department of Biological Engineering, Inha University, Inchon 402-751, Korea|
|Title||Negative Role of wblA in Response to Oxidative Stress in Streptomyces coelicolor|
J. Microbiol. Biotechnol.2012 ; 22(6):
|Abstract||In this study, we analyzed the oxidative stress response of
wblA (whiB-like gene A, SCO3579), which was previously
shown to be a global antibiotic down-regulator in Streptomyces
coelicolor. Ever since a WblA ortholog named WhcA in
Corynebacterium glutamicum was found to play a negative
role in the oxidative stress response, S. coelicolor wblA has
been proposed to have a similar effect. A wblA-deletion
mutant exhibited a less sensitive response to oxidative
stress induced by diamide present in solid plate culture.
Using real-time RT-PCR analysis, we also compared the
transcription levels of oxidative stress-related genes,
including sodF, sodF2, sodN, trxB, and trxB2, between S.
coelicolor wild type and a wblA-deletion mutant in the
presence or absence of oxidative stress. Target genes were
expressed higher in the wblA-deletion mutant compared
with wild type, both in the absence and presence of oxidative
stress. Moreover, expression of these target genes in S.
coelicolor wild type was stimulated only in the presence of
oxidative stress, suggesting that WblA plays a negative
role in the oxidative stress response of S. coelicolor, similar
to that of C. glutamicum WhcA, through the transcriptional
regulation of oxidative stress-related genes.|
|Keywords||wblA, Streptomyces coelicolor, oxidative stress|
Arner, E. S. and A. Holmgren. 2000. Physiological functions of thioredoxin and thioredoxin reductase. Eur. J. Biochem. 267:6102-6109.
Choi, S. U., C. K. Lee, Y. I. Hwang, H. Kinoshita, and T. Nihira. 2004. Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin B1 producer. Arch. Microbiol. 181: 294-298.
Choi, W. W., S. D. Park, S. M. Lee, H. B. Kim, Y. Kim, and H. S. Lee. 2008. The whcA gene plays a negative role in oxidative stress response of Corynebacterium glutamicum. FEMS Microbiol. Lett. 290: 32-38.
Chung, H. J., E. J. Kim, B. Suh, J. H. Choi, and J. H. Roe. 1999. Duplicate genes for Fe-containing superoxide dismutase in Streptomyces coelicolor A3(2). Gene 231: 87-93.
Crack, J. C., C. D. den Hengst, P. Jakimowicz, S. Subramanian, M. K. Jhonson, M. J. Buttner, et al. 2009. Characterization of [4Fe-4S]-containing and cluster-free forms of Streptomyces WhiD. Biochemistry 48: 12252-12264.
Crack, J. C., L. J. Smith, M. R. Stapleton, J. Peck, N. J. Watmough, M. J. Buttner, et al. 2011. Mechanistic insight into the nitrosylation of the [4Fe-4S] cluster of WhiB-like protein. J. Am. Chem. Soc. 133: 1112-1121.
Davis, N. K. and K. F. Chater. 1992. The Streptomyces coelicolor whiB gene encodes a small transcription factor-like protein dispensable for growth but essential for sporulation. Mol. Gen. Genet. 232: 351-358.
den Hengst, C. D. and M. J. Buttner. 2008. Redox control in actinobacteria. Biochem. Biophys. Acta 1780: 1201-1216.
Goldsworthy, K. F., B. Gust, S. Mouz, G. Chandra, K. C. Findlay, and K. F. Chater. 2011. The actinobacteria-specific gene wblA controls major development transition in Streptomyces coelicolor A3(2). Microbiology 157: 1312-1328.
Gladyshev, V. N. 2001. Thioredoxin and peptide methionine sulfoxide reductase: Convergence of similar structure and function in distinct structural folds. Protein 46: 149-152.
Jakimowicz, P., M. R. Cheesman, W. R. Bishai, K. F. Chater, A. J. Thomson, and M. J. Buttner. 2005. Evidence that the Streptomyces developmental protein WhiD, a member of the WhiB family, binds a [4Fe-4S] cluster. J. Biol. Chem. 280:8309-8315.
Kang, S. H., J. Huang, H. N. Lee, Y. A. Hur, S. N. Cohen, and E. S. Kim. 2007. Interspecies DNA microarray analysis identifies WblA as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces. J. Bacteriol. 189: 4315-4319.
Kim, E. J., H. J. Chung, B. Suh, Y. C. Hah, and J. H. Roe. 1998. Expression and regulation of the sodF gene encoding iron- and zinc-containing superoxide dismutase in Streptomyces coelicolor Miller. J. Bacteriol. 180: 2014-2020.
Kim, E. J., H. P. Kim, Y. C. Hah, and J. H. Roe. 1996. Differential expression of superoxide dismutases containing Ni and Fe/Zn in Streptomyces coelicolor. Eur. J. Biochem. 241:178-185.
Kim, T. H., J. S. Park, H. J. Kim, Y. Kim, P. Kim, and H. S. Lee. 2005. The whcE gene of Corynebacterium glutamicum is important for survival following heat and oxidative stress. Biochem. Biophys. Res. Commun. 337: 757-764.
Mustacich, D. and G. Powis. 2000. Thioredoxin reductase. Biochem. J. 346: 1-8.
Paget, M. S., J. G. Kang, J. H. Roe, and M. J. Buttner. 1998. SigmaR, an RNA polymerase sigma factor that modulates expression of the thioredoxin system in response to oxidative stress in Streptomyces coelicolor A3(2). EMBO J. 19: 57765782.
Park, J. S., S. Shin, E. S. Kim, P. Kim, Y. Kim, and H. S. Lee. 2011. Identification of SpiA that interacts with Corynebacterium glutamicum WhcA using a two-hybrid system. FEMS Microbiol. Lett. 322: 8-14.
Singh, A., L. Guidry, K. V. Narasimhulu, D. Mai, J. Trombley, K. E. Redding, et al. 2007. Mycobacterium tuberculosis WhiB3 responds to O2 and nitric oxide via its [4Fe-4S] cluster and is essential for nutrient starvation survival. Proc. Natl. Acad. Sci. USA 104: 11562-11567.
Soliveri, J. A., J. Gomez, W. R. Bishai, and K. F. Chater. 2000. Multiple paralogous genes related to the Streptomyces coelicolor developmental regulatory gene whiB are present in Streptomyces and other actinomycetes. Microbiology 146: 333-343.