Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
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2012 ; 22(12): 1605~1612

AuthorEun-Kyung Jang, Ihsan Ullah, Jong-Hui Lim, In-Jung Lee, Jong-Guk Kim, Jae-Ho Shin
AffiliationSchool of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea,Food and Biological Resources Examination Division, Korean Intellectual Property Office, Daejeon 302-701, Korea
TitlePhysiological and Molecular Characterization of a Newly Identified Entomopathogenic Bacteria, Photorhabdus temperata M1021
PublicationInfo J. Microbiol. Biotechnol.2012 ; 22(12): 1605~1612
AbstractThe present study concerned the identification and characterization of a novel bacterial strain isolated from entomopathogenic nematodes collected from different regions in Korea. The bacterial isolate M1021 was Gramnegative, bioluminescent, and produced red colonies on MacConkey agar medium. A rod-shaped structure was confirmed by the electron micrograph. Fatty acid composition was analyzed by using the Sherlock MIDI system. The identification was further supported by 16S rDNA sequence analysis, which revealed 96-99% sequence homology with strains of Photorhabdus temperata. The location of the isolated strain of P. temperata in the phylogenetic tree was confirmed and it was named P. temperata M1021. P. temperata M1021 exhibited catalase, protease, and lipase activities when grown on appropriate media supplemented with respective substrates. The culture of P. temperata M1021 exhibited insecticidal activity against the larvae of Galleria mellonella and the activity was the highest after 3-4 days of cultivation with agitating at 28oC under 220 rpm. Antibacterial activity was also observed against Salmonella Typhimurium KCTC 1926 and Micrococcus luteus KACC 10488.
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KeywordsAntibacterial activity, Entomopathogenic, Galleria mellonella, Insecticidal toxicity, Photorhabdus temperata
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