Journal of Microbiology and Biotechnology
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Journal of Microbiology and Biotechnology
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2012 ; 22(11): 1540~1548

AuthorK. Praveen, K. Y. Usha, Buddolla Viswanath, B. Rajasekhar Reddy
AffiliationDepartment of Microbiology, Sri Krishnadevaraya University, Anantapur-515 055, Andhra Pradesh, India
TitleKinetic Properties of Manganese Peroxidase from the Mushroom Stereum ostrea and its Ability to Decolorize Dyes
PublicationInfo J. Microbiol. Biotechnol.2012 ; 22(11): 1540~1548
AbstractManganese peroxidase (MnP) was isolated from the culture filtrate of the wood log mushroom Stereum ostrea (S. ostrea), grown on Koroljova medium, and then purified by ammonium sulfate [70% (w/v)] fractionation, DEAEcellulose anion exchange chromatography, and Sephadex G-100 column chromatography, with an attainment of 88.6-fold purification and the recovery of 22.8% of initial activity. According to SDS-PAGE the molecular mass of the MnP was 40 kDa. The optimal pH and temperature were found to be 4.5 and 35oC, respectively. The enzyme was stable even after exposure to a pH range of 4.5 to 6.0, and at temperatures of up to 35oC at a pH of 4.5 for 1h. The Km and Vmax values for the substrate phenol red were found to be 8 μm and 111.14 U/mg of protein, respectively. The MnP also oxidized other substrates such as guaiacol, DMP, and veratryl alcohol. Sodium azide, EDTA, SDS, Cu2+, and Fe2+, at 1-5 mM, strongly inhibited enzyme activity, whereas Ca2+ and Zn2+ increased enzyme activity. The participation of the purified enzyme in the decolorization of dyes suggests that S. ostrea manganese peroxidase could be effectively employed in textile industries.
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Keywordspurification, characterization, Stereum ostrea, kinetics, manganese peroxidase, dye decolorization
References
  1. Arora, D. S. and P. K. Gill. 2005. Production of ligninolytic enzymes by Phlebia floridensis. World J. Microbiol. Biotechnol. 21: 1021-1028.
    CrossRef
  2. Baborova, P., M. Moder, P. Baldrian, K. Cajthamlova, and T. Cajthamlova. 2006. Purification of a new manganese peroxidase of the white-rot fungus Irpex lacteus and degradation of polycyclic aromatic hydrocarbons by the enzyme. Res. Microbiol. 248253.
    Pubmed CrossRef
  3. Bermek, H., H. Yazici, H. Ozturk, C. Tamerler, H. C. Jung, K. C. Li, et al. 2004. Purification and characterization of manganese peroxidase from wood-degrading fungus Trichophyton rubrum LSK-27. Enzyme Microb. Technol. 35: 87-92.
    CrossRef
  4. Bonnarme, P. and T. W. Jeffries. 1990. Regulation of lignin peroxidases and manganese-dependent peroxidases from lignindegrading white-rot fungi. Appl. Environ. Microbiol. 56: 210217.
    Pubmed Pubmed Central
  5. Cai, Y., W. U. Huiguand, X. Liao, Y. Ding, J. Sun, and D. Zhang. 2010. Purification and characterization of novel manganese peroxidase from Rhizoctonia sp. SYBC-M3. Biotechnol. Bioproc. Eng. 1016-1021.
  6. Cheng, X., B. R. Jia, R. Li, S. Q. Zhu, W. Z. Tang, and X. D. Li. 2007. Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme. Enzyme Microb. Technol. 41: 258264.
    CrossRef
  7. Christian, C. C., R. Shrivastava, C. Novotny, and B. R. M. Vyas. 2003. Decolorization of sulfonaphthalein dyes by manganese peroxidase activity of the white-rot fungus Phanerochaete chrysosporium. Folia Microbiol. 6: 771-774.
    CrossRef
  8. Couto, S. R., D. Moldes, and A. Sanroman. 2006. Optimum stability conditions of pH and temperature for ligninase and manganesedependent peroxidase from Phanerochaete chrysosporium. Application to in vitro decolorization of Poly R-478 by MnP. World J. Microbiol. Biotechnol. 22: 607-612.
    CrossRef
  9. Fakoussa, R. M. and P. J. Hofrichter. 1999. In vivo-decolorization of coal-derived humic acids by laccase-excreting fungus Trametes versicolor. Appl. Microbiol. Biotechnol. 52: 60-65.
    CrossRef
  10. Giardina, P., G. Palmieri, B. Fontanella, V. Rivieccio, and G. Sannia. 2003. Manganese peroxidase isoenzymes produced by Pleurotus ostreatus grown on wood sawdust. Arch. Biochem. Biophys. 376: 171-179.
    Pubmed CrossRef
  11. Glenn, J. K. and M. H. Gold. 1985. Purification and characterization of an extracellular Mn(II)-dependent peroxidase from the lignin degrading basidiomycetes Phanerochaete chrysosporium. Arch. Biochem. Biophys. 242: 329.
    CrossRef
  12. Gold, M. H. and J. K. Glenn. 1998. Manganese peroxidase of Phanerochaete chrysosporium, pp. 258-264. In W. A. Wood and S. T. Kellog (eds.). Methods Enzymology. Academic Press San Diego, CA.
  13. Guerra, G., O. Domingues, M. Ramos-Leal, A. Manzano, M. I. Sanchez, I. Hernandez, et al. 2008. Production of laccase and manganese peroxidase by white-rot fungi from sugarcane bagasse in solid bed: Use for dyes decolorization. Sugar Tech. 10: 260-264.
    CrossRef
  14. Harris, R. Z., H. Wariishi, M. H. Gold, and P. R. O. Montellano. 1991. The catalytic site of manganese J. Biol. Chem. 14: 87518758.
  15. Hofrichter, M., T. Vares, M. Kalsi, S. Galkin, K. Scheibner, W. Fritsche, and A. Hatakka. 1999. Production of manganese peroxidase and organic acids and mineralization of 14C-labelled lignin (14C-DHP) during solid-state fermentation of wheat straw with the white-rot fungus Nematoloma frowardii. Appl. Environ. Microbiol. 65: 1864-1870.
    Pubmed Pubmed Central
  16. Hoshino, F., T. Kajino, H. Sugiyama, O. Assami, and H. Takahashi. 2002. Thermally stable and hydrogen peroxide tolerant manganese peroxidase (MnP) from Lenzites betulinus. FEBS Lett. 530:249-252.
    CrossRef
  17. Kamitsuji, H., Y. Honda, T. Watanabe, and M. Kuwahara. 2004. Production and induction of manganese peroxidase isozymes in a white-rot fungus Pleurotus ostreatus. Appl. Microbiol. Technol. 65: 287-294.
  18. Kanayama, N., S. Tohru, and K. Keiichi. 2002. Purification and characterization of an alkaline manganese peroxidase from Aspergillus terreus LD-1. J. Biosci. Bioeng. 93: 405-410.
    Pubmed
  19. Kariminiaae, H. R., A. Sakurai, and M. Sakakibara. 2007. Decolorization of synthetic dyes by a new manganese peroxidaseproducing white-rot fungus. Dyes Pigments 72: 157-162.
    CrossRef
  20. Koroljova-Skorobogat’Ko, O. V., E. V. Stepanova, V. P. Gavrilova, O. V. Morozovaa, N. V. Lubimova, A. N. Dzchafarova, et al. 1998. Purification and characterization of the constitutive form of laccase from the basidiomycetes Coriolus hirsutus and effect of inducers on laccase synthesis. Biotechnol. Appl. Biochem. 28: 47-54.
    Pubmed
  21. Kuwahara, M., J. K. Glenn, M. A. Morgan, and M. H. Gold. 1984. Separation and characterization of two extracellular H2O2dependent oxidases from lignolytic cultures of Phanerochaete chrysosporium. FEBS Lett. 169: 247-250.
    CrossRef
  22. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
    Pubmed CrossRef
  23. Machado, K. M. G., D. R. Matheus, and V. L. R. Bononi. 2005. Ligninolytic enzymes production and Remazol Brilliant Blue R decolorization by tropical Brazilian Basidiomycetes fungi. Braz. J. Microbiol. 36: 246-252.
    CrossRef
  24. Martinez, M. J., F. J. Fuiz-Duenas, F. Guillen, and A. T. Martinez. 1996. Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii. Eur. J. Biochem. 237: 424-432.
    Pubmed CrossRef
  25. Mielgo, I., C. M. Lopez, M. T. Moreira, G. Feijoo, and J. M. Lema. 2003. Oxidative degradation of azo dyes by manganese peroxidase under optimized condition. Biotechnol. Prog. 19:325-331.
    Pubmed CrossRef
  26. Paszczynski, A., V. B. Juynh, and R. L. Crawford. 1985. Enzymatic activities of an extracellular manganese-dependent peroxidase from Phanerochaete chrysosporium. FEMS Microbiol. Lett. 29: 37-41.
    CrossRef
  27. Pelaez, E., M. J. Martinez, and A. T. Martinez. 1995. Screening of 68 species of basidiomycetes for enzymes involved in lignin degradation. Mycol. Res. 99: 37-42.
    CrossRef
  28. Praveen, K., B. Viswanath, K. Y. Usha, H. Pallavi, G. V. Subba Reddy, M. Naveen, and B. Rajasekhar Redd. 2011. Lignolytic enzymes of a mushroom Stereum ostrea isolated from wood logs. Enz. Res. 1: 1-6.
    Pubmed CrossRef Pubmed Central
  29. Risna, R. and A. Suhirman. 2002. Ligninolytic enzyme production by Polyporaceae from Lombok, Indonesia. Fungal Divers. 9: 123-134.
  30. Sarkar, S., T. Angel, M. Martinez, and M. J. Martinez. 1997. Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus. Biochim. Biophys. Acta 1339: 23-30.
    CrossRef
  31. Schneegass, I., M. Hofrichter, K. Scheibner, and W. Fritsche. 1997. Purification of the main manganese peroxidase isoenzyme MnP2 from the white-rot fungus Nematoloma frowardii b19. Appl. Microbiol. Biotechnol. 48: 602-605.
    CrossRef
  32. Silva, E., M. S. F. Martins, and A. M. F. Milagres. 2008. Extraction of manganese peroxidase produced by Lentinula edodes. Bioresour. Technol. 9: 2471-2475.
    Pubmed CrossRef
  33. Snajdr, J. and P. Baldrian. 2007. Temperature affects the production, activity and stability of ligninolytic enzymes in Pleurotus ostreatus and Trametes versicolor. Folia Microbiol. 52: 498-502.
    CrossRef
  34. Sutherland, G. R. J. and S. D. Aust. 1996. The effects of calcium on the thermal stability and activity of manganese peroxidase. Arch. Biochem. Biophys. 332: 128-134.
    Pubmed CrossRef
  35. Taboada-Puig, R., T. Lu-Chau, M. T. Moreira, G. Feijoo, M. J. Martinez, and J. M. Lema. 2010. New strain of Bjerkandera sp. Production, purification and characterization of versatile peroxidase. World J. Microbiol. Biotechnol. 27: 115-122.
    CrossRef
  36. Urek, R. O. and N. K. Pazarlioglu. 2005. Enhanced production of manganese peroxidase by Phanerochaete chrysosporium. Braz. Arch. Biol. Technol. 6: 913-920.
  37. Vares, T., M. Kalsi, and A. Hatakka. 1995. Lignin peroxidases, manganese peroxidases and other ligninolytic enzymes produced by Phlebia radiata during solid state fermentation of wheat straw. Appl. Environ. Microbiol. 61: 3515-3520.
    Pubmed Pubmed Central
  38. Viswanath, B., M. S. Chandra, K. P. Kumar, and B. R. Reddy. 2008. Production and purification of laccase from Stereum ostrea and its ability to decolorize textile dyes. DBPBMB 2:19-25.
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