2012 ; 22(11):
|Author||Balachandrababu Malini Asha, Masilamani Revathi, Amit Yadav, Natarajan Sakthivel|
|Affiliation||Department of Biotechnology, School of Life Sciences, Pondicherry University, Kalapet, Puducherry 605014, India|
|Title||Purification and Characterization of a Thermophilic Cellulase from a Novel Cellulolytic Strain, Paenibacillus barcinonensis|
J. Microbiol. Biotechnol.2012 ; 22(11):
|Abstract||A novel bacterial strain, MG7, with high cellulase activity
was isolated and identified by morphological characteristics
and molecular phylogeny analysis as Paenibacillus
barcinonensis. Maximum production of cellulase by MG7
was observed at pH 7.0 and 35oC. The enzyme was purified
with a specific activity of 16.88 U/mg, the cellulase activity
was observed in a zymogram, and its molecular mass
(58.6 kDa) was confirmed by SDS-PAGE. The purified
enzyme showed maximum activity at pH 6.0 and 65oC and
degraded cellulosic substrates such as carboxy methyl
cellulose (CMC), Avicel, filter paper, and β-glucan. The
enzyme showed stability with 0.5% concentration of
various surfactants. The Km and Vmax of cellulase for CMC
and Avicel were found to be 0.459mg/ml and 10.46mg/ml/h,
and 1.01 mg/ml and 10.0 mg/ml/h, respectively. The high
catalytic activity and its stability to temperature, pH,
surfactants, and metal ions indicated that the cellulase
enzyme by MG7 is a good candidate for biotechnological
|Keywords||Cellulase, Paenibacillus, Kinetics, Avicelase, Specific activity|
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