Journal of Microbiology and Biotechnology
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2012 ; 22(10): 1388~1394

AuthorBin Liu, Ningning Zhang, Chao Zhao, Baixue Lin, Lianhui Xie, Yifan Huang
AffiliationNational Engineering Research Center of Juncao, Fuzhou, Fujian 350002, China,Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
TitleCharacterization of a Recombinant Thermostable Xylanase from Hot Spring Thermophilic Geobacillus sp. TC-W7
PublicationInfo J. Microbiol. Biotechnol.2012 ; 22(10): 1388~1394
AbstractA xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at 75oC and a pH of 8.2. The enzyme was active up to 95oC and showed activity over a wide pH range of 5.2 to 10.2. Additionally, the recombinant xylanase showed high thermostability and pH stability. More than 85% of the enzyme’s activity was retained after incubation at 70oC for 90 min at a pH of 8.2. The activity of the recombinant xylanase was enhanced by treatment with 10 mM enzyme inhibitors (DDT, Tween-20, 2-Me, or TritonX-100) and was inhibited by EDTA or PMSF. Its functionality was stable in the presence of Li+, Na+, and K+, but inhibited by Hg2+, Ni2+, Co2+, Cu2+, Zn2+, Pb2+, Fe3+, and Al3+. The functionality of the crude xylanase had similar properties to the recombinant xylanase except for when it was treated with Al2+ or Fe2+. The enzyme might be a promising candidate for various industrial applications such as the biofuel, food, and paper and pulp industries.
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KeywordsThermostable xylanase, Recombinant expression, Characterization, Stable pH, Geobacillus sp. TC-W7
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