Journal of Microbiology and Biotechnology
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2010 ; 20(10): 1403~1414

AuthorP. S. Beena, M. B. Soorej, K. K. Elyas, G.Bhat Sarita, M. Chandrasekaran
TitleAcidophilic tannase from marine Aspergillus awamori BTMFW032
PublicationInfo J. Microbiol. Biotechnol.2010 ; 20(10): 1403~1414
AbstractAspergillus awamori BTMFW032, isolated from sea water, produced tannase as extracellular enzyme under submerged culture conditions. Enzyme with a specific activity of 2761.89 IU/mg protein, a final yield of 0.51 %, and a purification fold of 6.32 was obtained after purification to homogeneity by ultrafiltration and gel filtration. SDS-PAGE analyses under non- reducing and reducing conditions yielded a single band of 230 kDa and 37.8 kDa, respectively, indicating presence of six identical monomers. pI of 4.4 and 8.02 % carbohydrate content in the enzyme were observed. Optimal temperature was 30ºC, although the enzyme was active at 5-80 ºC. Two pH optima, pH 2 and pH 8, were recorded and the enzyme was stable only at pH 2.0 for 24 h. Methylgallate recorded maximal affinity and Km and Vmax were recorded, respectively, as 1.9 X 10-3 M and 830 μmol/min.. Impact of several metal salts, solvents, surfactants, and typical enzyme inhibitors on tannase activity were determined to establish the novelty of the enzyme. Gene encoding tannase isolated from A. awamori is 1.232 kb and nucleic acid sequence analysis revealed an open reading frame consisting of 1122 bp (374 amino acids) of one stretch in -1 strand. In-silico analyses of gene sequences and comparison with reported sequences of other species of Aspergillus indicated that the acidophilic tannase from marine A. awamori is different from others.
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KeywordsAspergillus awamori, acidophilic tannase, characterization
References
1. Adachi, O., M. Watanabe, and H. Yamada. 1968 Studies on fungal tannase Part II. Physicochemical properties of the tannase from Aspergillus flavus. Agric. Biol. Chem. Studies on fungal tannase Part II. Physicochemical properties of the tannase from Aspergillus flavus. Agric. Biol. 32: 1079-1085


2. Aguilar, C. N., R. Rodriguez, G. Gutierrez-Sanchez, C. Augur, E. Favela-Torres, L. A. Prado-Barragan, A. Ramirez-Coronel, and J. C. Contreras-Esquivel. 2007 Microbial tannases: Advances and perspectives. Appl. Microbiol. Biotechnol. Microbial tannases: Advances and perspectives. Appl. Microbiol. 76: 47-59


3. Aoki, K., R. Shinke, and H. Nishira. 1976 Purification and some properties of yeast tannase. Agric. Biol. Chem. Purification and some properties of yeast tannase. Agric. Biol. 40: 79-85


4. Aoki, K., T. Tanaka, R. Shinke, and H. Nishira. 1979 Detection of tannase in polyacrylamide gels. J. Chromatogr. Detection of tannase in polyacrylamide gels. J. 170: 446-448


5. Bajpai, B. and S. Patil. 1996 Tannin acyl hydrolase (E.C. 3.1.1.20) activity of Aspergillus, Penicillium, Fusarium, and Trichoderma. World J. Microbiol. Biotechnol. Tannin acyl hydrolase (E.C. 3.1.1.20) activity of Aspergillus, Penicillium, Fusarium, and Trichoderma. World J. Microbiol. 12: 217-220


6. Barthomeuf, C., F. Regerat, and H. Pourrat. 1994 Production, purification and characterization of tannase from Aspergillus niger LCF8. J. Ferment. Technol. Production, purification and characterization of tannase from Aspergillus niger LCF8. J. Ferment. 77: 137-142


7. Battestin, V. and G. A. Macedo. 2007 Tannase production by Paecilomyces variotii. Bioresour. Technol. Tannase production by Paecilomyces variotii. Bioresour. 98: 1832-1837


8. Battestin, V., G. A. Macedo, and V. A. P. De Freitas. 2008 Hydrolysis of epigallocatechin gallate using a tannase from Paecilomyces variotii. Food Chem. Hydrolysis of epigallocatechin gallate using a tannase from Paecilomyces variotii. 108: 228-233


9. Belmares, R., J. C. Contreras-Esqcirel, R. Rodriguez-Herrera, A. Ramirez Coronel, and C. N. Aguilar. 2004 Microbial production of tannase: An enzyme with potential use in the food industry. Lebensm. Wiss. Technol. Microbial production of tannase: An enzyme with potential use in the food industry. Lebensm. Wiss. 37: 857-864


10. Bhardwaj, R., B. Singh, and T. K. Bhat. 2003. Purification and characterization of tannin acyl hydrolase from A. niger MTCC- 2425 J. Basic Microbiol. J. 43: 449-461


11. Boumans, H., M. C. van Gaalen, L. A. Grivell, and J. A. Berden. 1997 Differential inhibition of the yeast bc1 complex by phenanthrolines and ferroin. Implications for structure and catalytic mechanism. J. Biol. Chem. Differential inhibition of the yeast bc1 complex by phenanthrolines and ferroin. Implications for structure and catalytic mechanism. J. Biol. 272: 16753-16760


12. Bradford, M. M. 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254  : -


13. Brummer, W. and G. Gunzer. 1987 Laboratory techniques of enzyme recovery, pp. 260-264. In J. F. Kennedy (ed.). Biotechnology, Vol. 7a. VCH, Weinheim, Germany.  : -


14. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1956 Colorimetric method for determination of sugars and related substances. Anal. Chem. Colorimetric method for determination of sugars and related substances. Anal. 28: 350-356


15. Farias, G. M., C. Gorbea, J. R. Elkins, and G. J. Griffin. 1994 Purification, characterization, and substrate relationships of the tannase from Cryphonectria parasitica. Physiol. Mol. Plant Pathol. Purification, characterization, and substrate relationships of the tannase from Cryphonectria parasitica. Physiol. Mol. 44: 51-63


16. Gander, J. E. 1984 Gel protein stains: Glycoproteins. Methods Enzymol. Gel protein stains: Glycoproteins. 104: 447-449


17. García-Conesa, M. T., P. Ostergaard, S. Kauppinen, and G. Williamson. 2001 Hydrolysis of diethyl diferulates by a tannase from Aspergillus oryzae: Breaking cross-links between plant cell wall polymers. Carbohydr. Polym. Hydrolysis of diethyl diferulates by a tannase from Aspergillus oryzae: Breaking cross-links between plant cell wall polymers. Carbohydr. 44: 319-324


18. Hatamoto, O., T. Watarai, M. Kikuchi, K. Mizusawa, and H. Sekine. 1996 Cloning and sequencing of the gene encoding tannase and a structural study of the tannase subunit from Aspergillus oryzae. Gene 175: 215-221.  : -


19. Iibuchi, S., Y. Minoda, and K. Yamada. 1968 Studies on tannin acyl hydrolase of microorganisms Part III. Purification of the enzyme and some properties of it. Agric. Biol. Chem. Studies on tannin acyl hydrolase of microorganisms Part III. Purification of the enzyme and some properties of it. Agric. Biol. 32: 803-809


20. Iibuchi, S., Y. Minoda, and K. Yamada. 1972 Hydrolyzing pathway, substrate specificity and inhibition of tannin acyl hydrolase of Asp. orzo No.7. Agric. Biol. Chem. Hydrolyzing pathway, substrate specificity and inhibition of tannin acyl hydrolase of Asp. orzo No.7. Agric. Biol. 36: 1553-1562


21. Ikasari, L. and D. A. Mitchell. 1996 Leaching and characterization of Rhizopus oligosporus acid protease from solid-state fermentation. Enzyme Microb. Technol. Leaching and characterization of Rhizopus oligosporus acid protease from solid-state fermentation. Enzyme Microb. 19: 171-175


22. Jun, C. S., M. J. Yoo, W. Y. Lee, K. C. Kwak, M. S. Bae, W. T. Hwang, D. H. Son, and K. Y. Chai. 2007 Ester derivatives from tannase-treated prunioside A and their anti-inflammatory activities. Bull. Korean Chem. Soc. Ester derivatives from tannase-treated prunioside A and their anti-inflammatory activities. Bull. Korean Chem. 28: 73-76


23. Kar, B., R. Banerjee, and B. C. Bhattacharyya. 2003 Effect of additives on the behavioral properties of tannin acyl hydrolase. Process Biochem. Effect of additives on the behavioral properties of tannin acyl hydrolase. 38: 1285-1293


24. Kasieczka-Burnecka, M., K. Kuc, H. Kalinowska, M. Knap, and M. Turkiewicz. 2007 Purification and characterization of two cold-adapted extracellular tannin acyl hydrolases from an Antarctic strain Verticillium sp. P9. Appl. Microbiol. Biotechnol. Purification and characterization of two cold-adapted extracellular tannin acyl hydrolases from an Antarctic strain Verticillium sp. P9. Appl. Microbiol. 77: 77-89


25. Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680-685.  : -


26. Lee, S. B. and J. W. Taylor. 1990 Isolation of DNA from fungal mycelia and single spores, pp. 282-287. In M. A. Innis, D. H. Gefland, J. J. Sninsky, and T. J. White (eds.). PCR Protocols: A Guide to Methods and Applications. Academic Press San Diego, California.  : -


27. Lekha, P. K. and B. K. Lonsane. 1994 Comparative titres, location and properties of tannin acyl hydrolase produced by Aspergillus niger PKL 104 in solid-state, liquid surface and submerged fermentations. Process Biochem. Comparative titres, location and properties of tannin acyl hydrolase produced by Aspergillus niger PKL 104 in solid-state, liquid surface and submerged fermenta 29: 497-503


28. Lekha, P. K. and B. K. Lonsane. 1997 Production and application of tannin acyl hydrolase. State of the art. Adv. Appl. Microbiol. Production and application of tannin acyl hydrolase. State of the art. Adv. Appl. 44: 215-260


29. Lu, M. J. and C. Chen. 2007 Enzymatic tannase treatment of green tea increases in vitro inhibitory activity against Nnitrosation of dimethylamine. Process Biochem. Enzymatic tannase treatment of green tea increases in vitro inhibitory activity against Nnitrosation of dimethylamine. 42: 1285-1290


30. Lu, M. J. and C. Chen. 2008 Enzymatic modification by tannase increases the antioxidant activity of green tea. Food Res. Int. 41: 130-137


31. Machida, M., M. Sano, K. Tamano, Y. Terabayashi, N. Yamane, O. Hatamoto, et al. 2009 Genomics of industrial filamentous fungi, Aspergillus oryzae and Aspergillus awamori. The 17th Congress of the International Society for Human and Animal Mycology, Tokyo.  : -


32. Mahapatra, K., R. K. Nanda, S. S. Bag, R. Banerjee, A. Pandey, and G. Szakacs. 2005 Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa. Process Biochem. Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa. 40: 3251-3254


33. Marco, M. G., L. V. Rodríguez, E. L. Ramos, J. Renovato, M. A Cruz-Hernández, R. Rodríguez, J. Contreras, and C. N. Aguilar. 2009 A novel tannase from the xerophilic fungus Aspergillus niger GH1. J. Microbiol. Biotechnol. A novel tannase from the xerophilic fungus Aspergillus niger GH1. J. Microbiol. 19: 987-996


34. Naidu, R. B., N. Saisubramanian, D. Selvakumar, S. Janardhanan, and R. Puvanakrishnan. 2008 Partial purification of tannase from Aspergillus foetidus by aqueous two-phase extraction and its characterization. Curr. Trends Biotechnol. Pharm. Partial purification of tannase from Aspergillus foetidus by aqueous two-phase extraction and its characterization. Curr. Trends Biotechnol. 2: 201-207


35. Pohl, T. 1990 Concentration of proteins and removal of solutes. Methods Enzymol. 182: 68-83  : -


36. Raab, T., R. Bel-Rhlid, G. Williamson, C. E. Hansen, and D. Chaillot. 2007 Enzymatic galloylation of catechins in room temperature ionic liquids. J. Mol. Catal. B 44: 60-65.  : -


37. Raghukumar, C., U. Muraleedharan, V. R. Gaud, and R. Mishra. 2004 Xylanases of marine fungi of potential use for biobleaching of paper pulp. J. Ind. Microbiol. Biotechnol. Xylanases of marine fungi of potential use for biobleaching of paper pulp. J. Ind. Microbiol. 31: 433-441


38. Rajkumar, G. S. and S. C. Nandy. 1983 Isolation, purification, and some properties of Penicillium chrysogenium tannase. Appl. Environ. Microbiol. Isolation, purification, and some properties of Penicillium chrysogenium tannase. Appl. Environ. 46: 525-527


39. Sabu, A., G. S. Kiran, and A. Pandey. 2005 Purification and characterization of tannin acyl hydrolase from A. niger ATCC 16620. Food Technol. Biotechnol. Purification and characterization of tannin acyl hydrolase from A. niger ATCC 16620. Food Technol. 43: 133-138


40. Sehgal, A. C., R. Tompson, J. Cavanagh, and R. M. Kelly. 2002 Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1. Biotechnol. Bioeng. Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P 80: 784-793


41. Seth, M. and S. Chand. 2000 Biosynthesis of tannase and hydrolysis of tannins to gallic acid by Aspergillus awamori, optimization of process parameters. Process Biochem. Biosynthesis of tannase and hydrolysis of tannins to gallic acid by Aspergillus awamori, optimization of process parameters. 36: 39-44


42. Sharma, S., L. Agarwal, and R. K. Saxena. 2008 Purification, immobilization and characterization of tannase from Penicillium variable. Biores. Technol. Purification, immobilization and characterization of tannase from Penicillium variable. Biores. 99: 2544-2551


43. Sharma, S., T. K. Bhat, and R. K. Dawra. 1999 Isolation, purification and properties of tannase from Aspergillus niger van Tieghem. World J. Microbiol. Biotechnol. Isolation, purification and properties of tannase from Aspergillus niger van Tieghem. World J. Microbiol. 15: 673-677


44. Sharma, S., T. K. Bhat, and R. K. Dawra. 2000 A spectrophotometric method for assay of tannase using rhodonine. Anal. Biochem. A spectrophotometric method for assay of tannase using rhodonine. Anal. 278: 85-89


45. Singh, R., N. Gupta, V. K. Goswami, and R. Gupta. 2006 A simple activity staining protocol for lipases and esterases. Appl. Microbiol. Biotechnol. A simple activity staining protocol for lipases and esterases. Appl. Microbiol. 70: 679-682


46. Urbano, G., M. Lopez-Jurado, J. M. Porres, S. Frejnagel, E. Gomez-Villalva, J. Frias, C. Vidal-Valverde, and P. Aranda. 2007 Effect of treatment with α-galactosidase, tannase or a cell-wall-degrading enzyme complex on the nutritive utilization of protein and carbohydrates from pea (Pisum sativum L.) flour. J. Sci. Food Agric. Effect of treatment with α-galactosidase, tannase or a cell-wall-degrading enzyme complex on the nutritive utilization of protein and carbohydrates from pea (Pi 87: 1356-1363


47. Vallee, B. L. and D. D. Ulmer. 1972 Biochemical effects of mercury, cadmium and lead. Annu. Rev. Biochem. Biochemical effects of mercury, cadmium and lead. Annu. Rev. 41: 91-128


48. Van de Lagemaat, J. and D. L. Pyle. 2001 Solid-state fermentation and bioremediation: Development of a continuous process for the production of fungal tannase. Chem. Eng. J. Solid-state fermentation and bioremediation: Development of a continuous process for the production of fungal tannase. Chem. Eng. 84: 115-123


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