Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
Condition  Expression
When you enter More than two words, please use ‘and , or’ operation by means of putting ‘,(Comma Mark)’ between each word.

2009 ; 19(4): 397~402

AuthorG. S. Murugesan, M. Sathishkumar, R. Jayabalan, A. R. Binupriya, K. Swaminathan, S. E. Yun
AffiliationDepartment of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam-638401, Tamil Nadu, India,Division of Biotechnology, Department of Food Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju 561-756, Republic of Korea
TitleHepatoprotective and Curative Properties of Kombucha Tea Against Carbon Tetrachloride-Induced Toxicity
PublicationInfo J. Microbiol. Biotechnol.2009 ; 19(4): 397~402
AbstractKombucha tea (KT) is sugared black tea fermented with a symbiotic culture of acetic acid bacteria and yeasts, which is said to be tea fungus. KT is claimed to have various beneficial effects on human health, but there is very little scientific evidence available in the literature. In the present study, KT along with black tea (BT) and black tea manufactured with tea fungus enzymes (enzyme-processed tea, ET) were evaluated for hepatoprotective and curative properties against CCl4-induced toxicity, using male albino rats as an experimental model by analyzing aspartate transaminase, alanine transaminase, and alkaline phosphatase in plasma and malondialdehyde content in plasma and liver tissues. Histopathological analysis of liver tissue was also included. Results showed that BT, ET, and KT have the potential to revert the CCl4-induced hepatotoxicity. Among the three types of teas tried, KT was found to be more efficient than BT and ET. Antioxidant molecules produced during the fermentation period could be the reason for the efficient hepatoprotective and curative properties of KT against CCl4-induced hepatotoxicity.
Full-Text(PDF)
KeywordsKombucha tea, tea fungus, carbon tetrachloride
References
1. Blanc, P. J. 1996 Characterization of tea fungus metabolites. Biotechnol. Lett.  18: 139-142


2. Brent, J. A. and B. H. Rumack. 1993 Role of free radicals in toxic hepatic injury. Clin. Toxicol.  31: 173-196


3. Cancalon, P. F. and M. E. Parish. 1995 Changes in the chemical compositions of orange juice during growth of Saccharomyces cerevisiae and Gluconobacter oxydans. Food Microbiol.  12: 117-124


4. Chen, C. and B. Y. Liu. 2000 Changes in major components of tea fungus metabolites during prolonged fermentation. J. Appl. Microbiol.  89: 834-839


5. Chu, S. C. and C. Chen. 2006 Effect of origins and fermentation time on the antioxidant activities of Kombucha. Food Chem.  98: 502-507


6. CPSCEA guidelines for laboratory animal facility. 2003 Indian J. Pharmacol.  35: 257-274


7. Duncan, D. B. 1955 Multiple range “F” tests. Biometrics 11: 1-42.  : -


8. Elhalwagy, M. E. A., N. S. Darwish, and E. M. Zaher. 2008 Prophylactic effect of green tea polyphenols against liver and kidney injury induced by fenitrothion insecticide. Pesticide Biochem. Physiol.  91: 81-89


9. Fadhel, Z. A. and S. Amran. 2002 Effects of black tea extract on carbon tetrachloride induced lipid peroxidation in liver, kidneys and testes of rats. Phytother. Res. 16: S28-S32.  : -


10. Glenn, J. K., M. A. Morgan, M. B. Mayfield, M. Kuwahara, and M. H. Gold. 1983 An extracellular H2O2 requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete. Biochem. Biophys. Res. Commun.  114: 1077-1083


11. Gong, Y., C. Han, and J. Chen. 2000 Effect of tea polyphenols and tea pigments on the inhibition of precancerous liver lesions in rats. Nutr. Cancer 38: 81-86.  : -


12. He, P., Y. Noda, and K. Sugiyama. 2001 Green tea suppresses lipopolysaccharide-induced liver injury in D-galactosaminesensitized rats. J. Nutr.  131: 1560-1567


13. Hesseltine, C. W. 1965 A millenium of fungi, food and fermentation. Mycologia 5: 149-197.  : -


14. Humason, G. L. 1979 Animal Tissue Techniques, 4th Ed. Freeman, San Fransisco, CA  : -


15. Jadon, A., M. Bhadauria, and S. Shukla. 2007 Protective effect of Terminalia belerica Roxb. and gallic acid against carbon tetrachloride induced damage in albino rats. J. Ethnopharmol.  109: 214-218


16. Jain, A., M. S. L. Deb, A. Jain, S. P. Rout, V. B. Gupta, and K. L. Krishna. 2008 Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica oxb. leaves. J. Ethnopharmacol.  115: 61-66


17. Jayabalan, R., S. Marimuthu, and K. Swaminathan. 2007 Changes in content of organic acids and tea polyphenols during Kombucha fermentation. Food Chem.  102: 392-398


18. Jeon, T. I., S. G. Hwang, N. G. Park, Y. R. Jung, S. I. Shin, S. D. Choi, and D. K. Park. 2003 Antioxidative effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats. Toxicology 187: 67-73.  : -


19. King, E. and A. R. Armstong. 1934 Determination of serum and bile phosphastase activity. Can. Med. Assoc. J.  31: 376-378


20. Kumaravelu, P., D. P. Dakshinamoorthy, S. Subramaniam, H. Devaraj, and N. S. Devaraj. 1995 Effect of eugenol on drugmetabolizing enzymes of carbon tetrachloride-intoxicated rat liver. Biochem. Pharmacol.  49: 1703-1707


21. Lee, H. S., H. H. Kim, and S. K. Ku. 2008 Hepatoprotective effects of Artemisiae capillaris Herba and Picrorrhiza rhizoma combinations on carbon tetrachloride-induced subacute liver damage in rats. Nutr. Res.  28: 270-277


22. Lin, H. M., H. C. Tseng, C. J. Wang, J. J. Lin, C. W. Lo, and F. P. Chou. 2008 Hepatoprotective effects of Solanum nigrum Linn extract against CCl4-induced oxidative damage in rats. Chem. Biol. Inter.  171: 283-293


23. Nevin, K. G. and P. L. Vijayammal. 2005 Effect of Aerva lanata against hepatotoxicity of carbon tetrachloride in rats. Environ. Toxicol. Pharmacol.  20: 471-477


24. Nichans, W. G. and B. Samuelson. 1968 Formation of MDA from phospholipid arachidonate during microsomal lipid peroxidation. Eur. J. Biochem.  6: 126-130


25. Pauline, T., P. Dipti, B. Anju, S. Kavimani, S. K. Sharma, A. K. Kain, et al. 2001 Studies on toxicity, anti-stress and hepatoprotective properties of Kombucha tea. Biomed. Environ. Sci.  14: 207-213


26. Reitman, S. and S. A. Frankel, 1957 Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28: 56.  : -


27. Sai Ram, M., B. Anju, T. Pauline, P. Dipti, A. K. Kain, S. S. Mongia, et al. 2000 Effect of Kombucha tea on chromate(VI)- induced oxidative stress in albino rats. J. Ethnopharmacol.  71: 235-240


28. Yang, Y. S., T. H. Ahn, J. C. Lee, C. J. Moon, and S. H. Kim, 2008 Protective effects of pycnogenol on carbon tetrachlorideinduced hepatotoxicity in Sprague-Dawley rats. Food Chem. Toxicol.  46: 380-387


29. Zhen, M. C., Q. Wang, X. H. Huang, L. Q. Cao, X. L. Chen, K. Sun, Y. J. Liu, W. Li, and L. J. Zhang. 2007 Green tea polyphenol epigallocatechin-3-gallate inhibits oxidative damage and preventive effects on carbon tetrachloride-induced hepatic fibrosis. J. Nutr. Biochem.  18: 795-805


Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to jmb@jmb.or.kr
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by INFOrang.co., Ltd