Journal of Microbiology and Biotechnology
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2009 ; 19(1): 11~16

AuthorYang Seon Kim, Choi, Jin Hee, Yong-Hwan Lee
TitleFunctional Analysis of MCNA, a Gene Encoding a Catalytic Subunit of Calcineurin, in the Rice Blast Fungus Magnaporthe oryzae
PublicationInfo J. Microbiol. Biotechnol.2009 ; 19(1): 11~16
AbstractMagnaporthe oryzae, the causal agent of rice blast, forms a specialized infection structure, called an appressorium, which is crucial for penetration and infection of the host plant. Pharmacological data suggest that calcium/calmodulindependent signaling is involved in appressorium formation in this fungus. To understand the role of the calcium/ calmodulin-activated protein phosphatase on appressorium formation at the molecular level, MCNA, a gene encoding the catalytic subunit of calcineurin, was functionally characterized in M. oryzae. Transformants expressing sense/antisense RNA of MCNA exhibited significant reductions in mycelial growth, conidiation, appressorium formation, and pathogenicity. cDNA of MCNA functionally complemented a calcineurin disruptant strain (cmp1::LEU2 cmp2::HIS3) of Saccharomyces cerevisiae. These data suggest that calcineurin A plays important roles in signal transduction pathways involved in the infection-related morphogenesis and pathogenicity of M. oryzae.
KeywordsCalcineurin, Magnaporthe oryzae, calmodulin, protein phosphatase, sense/antisense, appressorium
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