Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
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2007 ; 17(11): 1890~1893

AuthorKim, Taesung, Jae-Hyung Ahn, Min-Kyeong Choi, Hang-Yeon Weon, Mi Sun Kim, Chi Nam Seong, Hong-Gyu Song, Jong-Ok Ka
TitleCloning and Expression of a Parathion Hydrolase Gene from a Soil Bacterium, Burkholderia sp. JBA3
PublicationInfo J. Microbiol. Biotechnol.2007 ; 17(11): 1890~1893
AbstractA bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to p-nitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes.
KeywordsParathion hydrolase gene, Burkholderia sp. JBA3, biodegradation
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