2020 ; 30(2):
|Author||Dong-Ho Chang, Jongoh Shin, Moon-Soo Rhee, Kyung-Ryang Park, Byung-Kwan Cho, Sung-Ki Lee, Byoung-Chan Kim|
|Affiliation||Metabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea,Department of Biological Science and Biotechnology, Hannam University, Daejeon 34054, Republic of Korea|
|Title||Vaginal Microbiota Profiles of Native Korean Women and Associations with High-Risk Pregnancy|
J. Microbiol. Biotechnol.2020 ; 30(2):
|Abstract||The vaginal microbiota may be important for pregnancy prognosis because vaginal dysbiosis
during pregnancy appears to be related to preterm birth (PTB) or pregnancy loss. Previous
reports have indicated that a Lactobacillus-poor microbial flora in the vagina and intrauterine
infection by diverse anaerobes ascending from the vagina are associated with undesirable
delivery outcomes. However, no research has involved the use of pyrosequencing analysis to
examine vaginal microbiota profiles or their potential associations with high-risk pregnancy in
Korean women. Vaginal swabs were collected from 500 Korean women for the identification of
community state types (CSTs). Of these, 137 samples were further analyzed using a Roche/454
GS Junior pyrosequencer. Three distinct CSTs were identified based on the dominant vaginal
microbes: CST I (Lactobacillus crispatus dominated), CST III (Lactobacillus iners dominated), and
CST IV (with diverse species of anaerobes). Twelve of the 67 pregnant women had undesirable
pregnancy outcomes (four miscarriages and eight PTBs). The dominant microbe in the vaginal
microbiota of women who gave birth at full-term was L. crispatus. In contrast, L. iners was the
dominant vaginal microbe in women who miscarried. Most (n = 6/8) vaginal microbiota
profiles of women who experienced PTB could be classified as CST IV, with diverse bacteria,
including anaerobic vaginal species. The present study provides valuable information
regarding the characteristics of the vaginal microbiota of Korean women related to high-risk
pregnancy. Investigation of the vaginal microbiotic structure in pregnant Korean women is
necessary to enable better prediction of adverse pregnancy outcomes.|
|Keywords||Vaginal microbiota, high risk pregnancy, preterm, miscarriage|
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