Journal of Microbiology and Biotechnology
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Journal of Microbiology and Biotechnology
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2020 ; 30(2): 248~258

AuthorDong-Ho Chang, Jongoh Shin, Moon-Soo Rhee, Kyung-Ryang Park, Byung-Kwan Cho, Sung-Ki Lee, Byoung-Chan Kim
AffiliationMetabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea,Department of Biological Science and Biotechnology, Hannam University, Daejeon 34054, Republic of Korea
TitleVaginal Microbiota Profiles of Native Korean Women and Associations with High-Risk Pregnancy
PublicationInfo J. Microbiol. Biotechnol.2020 ; 30(2): 248~258
AbstractThe vaginal microbiota may be important for pregnancy prognosis because vaginal dysbiosis during pregnancy appears to be related to preterm birth (PTB) or pregnancy loss. Previous reports have indicated that a Lactobacillus-poor microbial flora in the vagina and intrauterine infection by diverse anaerobes ascending from the vagina are associated with undesirable delivery outcomes. However, no research has involved the use of pyrosequencing analysis to examine vaginal microbiota profiles or their potential associations with high-risk pregnancy in Korean women. Vaginal swabs were collected from 500 Korean women for the identification of community state types (CSTs). Of these, 137 samples were further analyzed using a Roche/454 GS Junior pyrosequencer. Three distinct CSTs were identified based on the dominant vaginal microbes: CST I (Lactobacillus crispatus dominated), CST III (Lactobacillus iners dominated), and CST IV (with diverse species of anaerobes). Twelve of the 67 pregnant women had undesirable pregnancy outcomes (four miscarriages and eight PTBs). The dominant microbe in the vaginal microbiota of women who gave birth at full-term was L. crispatus. In contrast, L. iners was the dominant vaginal microbe in women who miscarried. Most (n = 6/8) vaginal microbiota profiles of women who experienced PTB could be classified as CST IV, with diverse bacteria, including anaerobic vaginal species. The present study provides valuable information regarding the characteristics of the vaginal microbiota of Korean women related to high-risk pregnancy. Investigation of the vaginal microbiotic structure in pregnant Korean women is necessary to enable better prediction of adverse pregnancy outcomes.
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KeywordsVaginal microbiota, high risk pregnancy, preterm, miscarriage
References
  1. Dethlefsen L, McFall-Ngai M, Relman DA. 2007. An ecological and evolutionary perspective on human-microbe mutualism and disease. Nature 449: 811-818.
    Pubmed CrossRef
  2. Seon-Kyun Kim, Robin B Guevarra, You-Tae Kim, Joongi Kwon, Hyeri Kim, Jae Hyoung Cho, et al. 2019. Role of probiotics in human gut microbiome-associated diseases. J. Microbiol. Biotechnol. 29: 1335-1340.
    Pubmed CrossRef
  3. Kau AL, Ahern PP, Griffin NW, Goodman AL, Gordon JI. 2011. Human nutrition, the gut microbiome and the immune system. Nature 474: 327-336.
    Pubmed CrossRef Pubmed Central
  4. Sungmi Choi, Yu-Jin Hwang, Min-Jeong Shin, Hana Yi. 2017. Difference in the gut microbiome between ovariectomyinduced obesity and diet-induced obesity. J. Microbiol. Biotechnol. 27: 2228-2236.
    Pubmed CrossRef
  5. Onderdonk, AB, Delaney ML, Fichorova RN. 2016. The human microbiome during bacterial vaginosis. Clin. Microbiol. Rev. 29: 223-238.
    Pubmed CrossRef Pubmed Central
  6. Ollberding NJ, Völgyi E, Macaluso M, Kumar R, Morrow C, Tylavsky FA, et al. 2016. Urinary microbiota associated with preterm birth: results from the conditions affecting neurocognitive development and learning in early childhood (CANDLE) study. PLoS One 11: e0162302.
    Pubmed CrossRef Pubmed Central
  7. Zhou X, Bent SJ, Schneider MG, Davis CC, Islam MR, Forney LJ. 2004. Characterization of vaginal microbial communities in adult healthy women using cultivationindependent methods. Microbiology 150: 2565-2573.
    Pubmed CrossRef
  8. DiGiulio DB, Callahan BJ, McMurdie PJ, Costello EK, Lyell DJ, Robaczewska A, et al. 2015. Temporal and spatial variation of the human microbiota during pregnancy. Proc. Natl. Acad. Sci. USA 112: 11060-11065.
    Pubmed CrossRef Pubmed Central
  9. Alioua S, Abdi A, Fhoula I, Bringel F, Boudabous A, Ouzari IH. 2016. Diversity of vaginal lactic acid bacterial microbiota in 15 algerian pregnant women with and without bacterial vaginosis by using culture independent method. J. Clin. Diagn. Res. 10: DC23-DC27.
    Pubmed CrossRef Pubmed Central
  10. Hammerschlag MR, Alpert S, Onderdonk AB, Thurston P, Drude E, McCormack WM, et al. 1978. Anaerobic microflora of the vagina in children. Am. J. Obstet. Gynecol. 131: 853-856.
    CrossRef
  11. Farage M, Maibach H. 2006. Lifetime changes in the vulva and vagina. Arch. Gynecol. Obstet. 273: 195-202.
    Pubmed CrossRef
  12. Gupta S, Kumar N, Singhal N, Kaur R, Manektala U. 2006. Vaginal microflora in postmenopausal women on hormone replacement therapy. Indian J. Pathol. Bacteriol. 49: 457-461.
  13. Cone RA. 2014. Vaginal microbiota and sexually transmitted infections that may influence transmission of cell-associated HIV. J. Infect. Dis. 210: S616-S621.
    Pubmed CrossRef Pubmed Central
  14. Hyman RW, Fukushima M, Jiang H, Fung E, Rand L, Johnson B, et al. 2013. Diversity of the vaginal microbiome correlates with preterm birth. Reprod. Sci. 21: 32-40.
    Pubmed CrossRef Pubmed Central
  15. Petricevic L, Domig KJ, Nierscher FJ, Sandhofer MJ, Fidesser M, Krondorfer I, et al. 2014. Characterisation of the vaginal Lactobacillus microbiota associated with preterm delivery. Sci. Rep. 4: 5136.
    Pubmed CrossRef Pubmed Central
  16. Hay PE, Lamont RF, Taylor-Robinson D, Morgan DJ, Ison C, Pearson J. 1994. Abnormal bacterial colonisation of the genital tract and subsequent preterm delivery and late miscarriage. BMJ 308: 295-298.
    Pubmed CrossRef Pubmed Central
  17. Llahi-Camp JM, Rai R, Ison C, Regan L, Taylor-Robinson D. 1996. Association of bacterial vaginosis with a history of second trimester miscarriage. Hum. Reprod. 11: 1575-1578.
    Pubmed CrossRef
  18. Martius J, Eschenbach DA. 1990. The role of bacterial vaginosis as a cause of amniotic fluid infection, chorioamnionitis and prematurity. Arch. Gynecol. Obstet. 247: 1-13.
    Pubmed CrossRef
  19. Watts DH, Krohn MA, Hillier SL, Eschenbach DA. 1990. Bacterial vaginosis as a risk factor for post-cesarean endometritis. Obstet. Gynecol. 75: 52-58.
  20. Romero R, Hassan SS, Gajer P, Tarca AL, Fadrosh DW, Bieda J, et al. 2014. The vaginal microbiota of pregnant women who subsequently have spontaneous preterm labor and delivery and those with a normal delivery at term. Microbiome 2: 18. doi: 10.1186/2049-2618-2-18.
    Pubmed CrossRef Pubmed Central
  21. Borgdorff H , van der Veer C , van Houdt R, A lberts C J, d e Vries HJ, Bruisten SM, et al. 2017. The association between ethnicity and vaginal microbiota composition in Amsterdam, the Netherlands. PLoS One 12: e0181135.
    Pubmed CrossRef Pubmed Central
  22. Haque MM, M erchant M, Kumar PN, D utta A , Mande SS. 2017. First-trimester vaginal microbiome diversity: a potential indicator of preterm delivery risk. Sci. Rep. 7:16145.
    Pubmed CrossRef Pubmed Central
  23. Callahan BJ, DiGiulio DB, Goltsman DSA, Sun CL, Costello EK, Jeganathan P, et al. 2017. Replication and refinement of a vaginal microbial signature of preterm birth in two racially distinct cohorts of US women. Proc. Natl. Acad. Sci. USA 114: 9966-9971.
    Pubmed CrossRef Pubmed Central
  24. Chaban B, Links MG, Jayaprakash TP, Wagner EC, Bourque DK, Lohn Z, et al. 2014. Characterization of the vaginal microbiota of healthy Canadian women through the menstrual cycle. Microbiome 2: 23.
    Pubmed CrossRef Pubmed Central
  25. Chun J, Kim KY, Lee J-H, Choi Y. 2010. The analysis of oral microbial communities of wild-type and toll-like receptor 2deficient mice using a 454 GS FLX Titanium pyrosequencer. BMC Microbiol. 10: 101.
    Pubmed CrossRef Pubmed Central
  26. Hur M, Kim Y, Song HR, Kim JM, Choi YI, Yi H. 2011. Effect of genetically modified Poplars on soil microbial communities during the phytoremediation of waste mine tailings. Appl. Environ. Microbiol. 77: 7611-7619.
    Pubmed CrossRef Pubmed Central
  27. Kim BS, Kim JN, Yoon SH, Chun J, Cerniglia CE. 2012. Impact of enrofloxacin on the human intestinal microbiota revealed by comparative molecular analysis. Anaerobe 18: 310-320.
    Pubmed CrossRef
  28. Huber T, Faulkner G, Hugenholtz P. 2004. Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Bioinformatics 20: 2317-2319.
    Pubmed CrossRef
  29. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, et al. 2012. Introducing Eztaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62: 716-721.
    Pubmed CrossRef
  30. Hamady M, Lozupone C, Knight R. 2010. Fast UniFrac:facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and PhyloChip data. ISME J. 4: 17-27.
    Pubmed CrossRef Pubmed Central
  31. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. 2010. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods 7: 335–336.
    Pubmed CrossRef Pubmed Central
  32. Caporaso JG, Bittinger K, Bushman FD, DeSantis TZ, Andersen GL, Knight R. 2010. PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 26: 266-267.
    Pubmed CrossRef Pubmed Central
  33. Roberge S, Villa P, Nicolaides K, Giguère Y, Vainio M, Bakthi A, et al. 2012. Early administration of low-dose aspirin for the prevention of preterm and term preeclampsia: a systematic review and meta-analysis. Fetal Diagn. Ther. 31: 141-146
    Pubmed CrossRef
  34. Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SS, McCulle SL, et al. 2011. Vaginal microbiome of reproductive-age women. Proc. Natl. Acad. Sci. USA 108: 4680-4687.
    Pubmed CrossRef Pubmed Central
  35. Redelinghuys MJ, Ehlers MM, D reyer AW, L ombaard H A , Kock MM. 2013. Comparison of the new Mycofast Revolution assay with a molecular assay for the detection of genital mycoplasmas from clinical specimens. BMC Infect. Dis. 13: 453.
    Pubmed CrossRef Pubmed Central
  36. Kwak DW, Hwang HS, Kwon JY, Park YW, Kim YH. 2014. Co-infection with vaginal Ureaplasma urealyticum and Mycoplasma hominis increases adverse pregnancy outcomes in patients with preterm labor or preterm premature rupture of membranes. J. Matern. Fetal Neonatal Med. 27: 333-337.
    Pubmed CrossRef
  37. Cox C, Saxena N, Watt AP, Gannon C, McKenna JP, Fairley DJ, et al. 2016. The common vaginal commensal bacterium Ureaplasma parvum is associated with chorioamnionitis in extreme preterm labor. J. Matern. Fetal Neonatal Med. 29: 3646-3651.
    Pubmed CrossRef
  38. Tabatabaei N, Eren AM, Barreiro LB, Yotova V, Dumaine A, Allard C, Fraser WD. 2018. Vaginal microbiome in early pregnancy and subsequent risk of spontaneous preterm birth: a case-control study. BJOG 126: 349-358.
    Pubmed CrossRef
  39. Bretelle F, Rozenberg P, Pascal A, Favre R, Bohec C, Loundou A, et al. 2015. High Atopobium vaginae and Gardnerella vaginalis vaginal loads are associated with preterm birth. Clin. Infect. Dis. 60: 860-867.
    Pubmed CrossRef
  40. Freitas AC, Bocking A, Hill JE, Money DM; VOGUE Research Group. 2018. Increased richness and diversity of the vaginal microbiota and spontaneous preterm birth. Microbiome 6: 117.
    Pubmed CrossRef Pubmed Central
  41. Freitas AC, Chaban B, Bocking A, Rocco M, Yang S, Hill JE, et al. 2017. The vaginal microbiome of pregnant women is less rich and diverse, with lower prevalence of Mollicutes, compared to non-pregnant women. Sci. Rep. 7: 9212.
    Pubmed CrossRef Pubmed Central
  42. Lee JE, Lee S, Lee H, Song YM, Lee K, Han MJ, et al. 2013. Association of the vaginal microbiota with human papillomavirus infection in a Korean twin cohort. PLoS One 8: e63514.
    Pubmed CrossRef Pubmed Central
  43. Onderdonk AB, Delaney ML, Fichorova RN. 2016. The human microbiome during bacterial vaginosis. Clin. Microbiol. Rev. 29: 223-238.
    Pubmed CrossRef Pubmed Central
  44. Vitali B, Cruciani F, Picone G, Parolin C, Donders G, Laghi L. 2015. Vaginal microbiome and metabolome highlight specific signatures of bacterial vaginosis. Eur. J. Clin. Microbiol. Infect. Dis. 34: 2367-2376.
    Pubmed CrossRef
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