2019 ; 29(11):
|Author||In Sung Kim, Seung Ho Lee, Young Min Kwon, Bishnu Adhikari, Jeong A Kim, Da Yoon Yu, Gwang Il Kim, Jong Min Lim, Sung Hak Kim, Sang Suk Lee, Yang Soo Moon, In Soon Choi, Kwang Keun Cho|
|Affiliation||Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea|
|Title||Oral Administration of β-Glucan and Lactobacillus plantarum Alleviates Atopic Dermatitis-Like Symptoms|
J. Microbiol. Biotechnol.2019 ; 29(11):
|Abstract||Atopic dermatitis (AD) is a chronic inflammatory skin disease of mainly infants and children.
Currently, the development of safe and effective treatments for AD is urgently required. The
present study was conducted to investigate the immunomodulatory effects of yeast-extracted
β-1,3/1,6-glucan and/or Lactobacillus plantarum (L. plantarum) LM1004 against AD-like
symptoms. To purpose, β-1,3/1,6-glucan and/or L. plantarum LM1004 were orally
administered to AD-induced animal models of rat (histamine-induced vasodilation) and
mouse (pruritus and contact dermatitis) exhibiting different symptoms of AD. We then
investigated the treatment effects on AD-like symptoms, gene expression of immune-related
factors, and gut microbiomes. Oral administration of β-1,3/1,6-glucan (0.01 g/kg initial body
weight) and/or 2 × 1012 cells/g L. plantarum LM1004 (0.01 g/kg initial body weight) to ADinduced
animal models showed significantly reduced vasodilation in the rat model, and
pruritus, edema, and serum histamine in the mouse models (p < 0.05). Interestingly, β-1,3/1,6-
glucan and/or L. plantarum LM1004 significantly decreased the mRNA levels of Th2 and Th17
cell transcription factors, while the transcription factors of Th1 and Treg cells, galactin-9,
filaggrin increased, which are indicative of enhanced immunomodulation (p < 0.05).
Moreover, in rats with no AD induction, the same treatments significantly increased the
relative abundance of phylum Bacteroidetes and the genus Bacteroides. Furthermore, bacterial
taxa associated with butyrate production such as, Lachnospiraceae and Ruminococcaceae at
family, and Roseburia at genus level were increased in the treated groups. These findings
suggest that the dietary supplementation of β-1,3/1,6-glucan and/or L. plantarum LM1004 has
a great potential for treatment of AD as well as obesity in humans through mechanisms that
might involve modulation of host immune systems and gut microbiota.|
|Keywords||Gut microbiota, Immunomodulation, Th1 cells, Th2 cells, treg cells|
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