2018 ; 28(7):
|Author||Xiaojian Ma, Chongde Wu, Jun Huang, Rongqing Zhou, Bi Shi|
|Affiliation||National Engineering Laboratory of Clean Technology for Leather Manufacture, Sichuan University, Chengdu 610065, China,College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China|
|Title||Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing|
J. Microbiol. Biotechnol.2018 ; 28(7):
|Abstract||The aim of this study was to investigate the microbial community of three tannery wastewater
treatment plants (WWTPs) involved in nitrification by Illumina MiSeq sequencing. The results
showed that highly diverse communities were present in tannery wastewater. A total of six
phyla, including Proteobacteria (37-41%), Bacteroidetes (6.04-16.80), Planctomycetes (3.65-
16.55), Chloroflexi (2.51-11.48), Actinobacteria (1.91-9.21), and Acidobacteria (3.04-6.20),
were identified as the main phyla, and Proteobacteria dominated in all the samples. Within
Proteobacteria, Beta-proteobacteria was the most abundant class, with the sequence
percentages ranging from 9.66% to 17.44%. Analysis of the community at the genus level
suggested that Thauera, Gp4, Ignavibacterium, Phycisphaera, and Arenimonas were the core
genera shared by at least two tannery WWTPs. A detailed analysis of the abundance of
ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) indicated that
Nitrosospira, Nitrosomonas, and Nitrospira were the main AOB and NOB in tannery wastewater,
respectively, which exhibited relatively high abundance in all samples. In addition, real-time
quantitative PCR was conducted to validate the results by quantifying the abundance of the
AOB and total bacteria, and similar results were obtained. Overall, the results presented in
this study may provide new insights into our understanding of key microorganisms and the
entire community of tannery wastewater and contribute to improving the nitrogen removal
|Keywords||Microbial community, nitrification, tannery wastewater, high-throughput sequencing|
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