Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
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2018 ; 28(1):

Authorhongyu wen, xin yin, zhenya yuan, xiuying wang, siting su
AffiliationSchool of Life Science, Jiangsu Normal University, Xuzhou, China
TitleComparative Analysis of Gut Microbial Communities in Children under 5 YearsOld with Diarrhea
PublicationInfo J. Microbiol. Biotechnol.2018 ; 28(1):
AbstractDiarrhea is a global disease with the high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples have been taken from healthy children without diarrhea and marked as the healthy control group; eight samples have been sampled from the children with diarrhea caused by dyspepsia and defined as thenon-infectious group, and ten samples have been taken from children with diarrhea induced by intestinal infections and identified as the infectious group. We detected the microbial communities of samples by usinghigh-throughput sequencing of 16S rRNA genes. The proportion of aerobic and facultative anaerobic microbesin samples ofthe infectious group was much higher than in the non-infectious group. In addition, the relative abundance of Enterococcus in the healthy control group was significantly higher than in thenon-infectious group and infectious group that can be used asa potential diagnostic biomarker for diarrhea.
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Keywordschildren diarrhea, gut microbial communities, high-throughput sequencing, 16S rRNA genes
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