2018 ; 28(4):
|Author||Hongyu Wen, Xin Yin, Zhenya Yuan, Xiuying Wang, Siting Su|
|Affiliation||School of Life Science, Jiangsu Normal University, Xuzhou, China|
|Title||Comparative Analysis of Gut Microbial Communities in Children under 5 Years Old with Diarrhea|
J. Microbiol. Biotechnol.2018 ; 28(4):
|Abstract||Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study,
25 fecal samples were collected from children under 5 years old. Seven samples had been
taken from healthy children without diarrhea and marked as the healthy control group; eight
samples had been sampled from children with diarrhea caused by dyspepsia and defined as
the non-infectious group; and ten samples had been taken from children with diarrhea
induced by intestinal infections and identified as the infectious group. We detected the
microbial communities of samples by using high-throughput sequencing of 16S rRNA genes.
The proportion of aerobic and facultative anaerobic microbes in samples of the infectious
group was much higher than in the non-infectious group. In addition, the relative abundance
of Enterococcus in the healthy control group was significantly higher than in the non-infectious
group and infectious group. This can be used as a potential diagnostic biomarker for diarrhea.|
|Keywords||Children diarrhea, gut microbial communities, high-throughput sequencing, 16S rRNA genes|
Van Niel CW, Feudtner C, Garrison MM, Christakis DA. 2002. Lactobacillus therapy for acute infectious diarrhea in children: a meta-analysis. Pediatrics 109: 678-684.
Walker CLF, Aryee MJ, Boschi-Pinto C, Black RE. 2012. Estimating diarrhea mortality among young children in low and middle income countries. PLoS One 7: e29151.
Qu M, Deng Y, Zhang X, Liu G, Huang Y, Lin C, et al. 2012. Etiology of acute diarrhea due to enteropathogenic bacteria in Beijing, China. J. Infect. 65: 214-222.
Colombara DV, Khalil AM, Rao PC, Troeger C, Forouzanfar MH, Riddle MS, et al. 2016. Chronic health consequences of acute enteric infections in the developing world. Am. J. Gastroenterol. 3: 12-24.
Harris VC, Armah G, Fuentes S, Korpela KE, Parashar U, Victor JC. 2017. The infant gut microbiome correlates significantly with rotavirus vaccine response in rural Ghana. J. Infect. Dis. 215: 34-41.
Kajbaf TZ, Shamsizadeh A, Kalvandi G, Macvandi M. 2013. Relative frequency of rotavirus and adenovirus among children aged 1-60 months hospitalized with acute diarrhoea in south-western, Iran. Jundishapur J. Microbiol. 6: 47-50.
Kiulia NM, Mwenda JM, Nyachieo A, Nyaundi JK, Steele AD, Taylor MB. 2007. Astrovirus infection in young Kenyan children with diarrhoea. J. Trop. Pediatr. 53: 206-209.
Castro TX, Cubel GRC, Fumian TM, Costa EM, Mello R, White PA. 2015. Detection and molecular characterization of caliciviruses (vesivirus and norovirus) in an outbreak of acute diarrhea in kittens from Brazil. Vet. J. 206: 115-117.
Randremanana R, Randrianirina F, Gousseff M, Dubois N, Razafindratsimandresy R, Hariniana ER, et al. 2012. Casecontrol study of the etiology of infant diarrheal disease in 14 districts in Madagascar. PLoS One 7: e44533.
Majowicz S E, Musto J, Scallan E, Angulo FJ, Kirk M, O’Brien SJ, et al. 2010. The global burden of nontyphoidal Salmonella gastroenteritis. Clin. Infect. Dis. 50: 882-889.
Tadesse G. 2014. Prevalence of human salmonellosis in Ethiopia: a systematic review and meta-analysis. BMC Infect. Dis. 14: 88.
Striepen B. 2013. Parasitic infections: time to tackle cryptosporidiosis. Nature 503: 189-191.
Tsuchiaka S, Masuda T, Sugimura S, Kobayashi S, Komatsu N, Nagai M, et al. 2016. Development of a novel detection system for microbes from bovine diarrhea by real-time PCR. J. Vet. Med. Sci. 78: 383-389.
Ley RE, Peterson DA, Gordon JI. 2006. Ecological and evolutionary forces shaping microbial diversity in the human intestine. Cell 124: 837-848.
Huang Z, Li X, Wang L, Shao Z. 2016. Changes in the intestinal bacterial community during the growth of white shrimp, Litopenaeus vannamei. Aquac. Res. 47: 1737-1746.
Tap J, Mondot S, Levenez F, Pelletier E, Carbon C, Furet JP, et al. 2009. Towards the human intestinal microbiota phylogenetic core. Environ. Microbiol. 11: 2574-2584.
Christensen EG, L icht TR, Leser TD, Bahl MI . 2014. Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats. BMC Res. Notes 7: 660.
Hao WL, Lee YK. 2004. Microflora of the gastrointestinal tract: a review. Methods Mol. Biol. 268: 491-502.
Reis RS, Horn F. 2010. Enteropathogenic Escherichia coli, Samonella, Shigella and Yersinia: cellular aspects of hostbacteria interactions in enteric diseases. Gut Pathog. 2: 8.
Haque R, Neville LM, Hahn P, Petri WAP Jr. 1995. Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits. J. Clin. Microbiol. 33: 2558-2561.
Kirby A, Gurgel RQ, Dove W, Vieira SCF, Cunliffe NA, Cuevas LE. 2010. An evaluation of the RIDASCREEN and IDEIA enzyme immunoassays and the RIDAQUICK immunochromatographic test for the detection of norovirus in faecal specimens. J. Clin. Virol. 49: 254-257.
Mardis ER. 2008. Next-generation DNA sequencing methods. Annu. Rev. Genomics Hum. Genet. 9: 387-402.
Novais RC, Thorstenson YR. 2011. The evolution of pyrosequencing for microbiology: from genes to genomes. J. Microbiol. Methods 86: 1-7.
Glimcher LH. 2010. Homeostasis and inflammation in the intestine. Cell 140: 859-870.
Emonet S, Lazarevic V, Pugin J, Schrenzel J, Ruppé E. 2017. Clinical metagenomics for the diagnostic of hospitalacquired infections: promises and hurdles. Am. J. Respir. Crit. Care Med. 196: 1617-1618.
Wang Y, Wang Y, Wang Y, Murray CK, Hamblin MR, Hooper DC, et al. 2017. Antimicrobial blue light inactivation of pathogenic microbes: state of the art. Drug Resist. Updat. 33-35: 1-22.
Kumar GD, Ravi S, Micallef SA, Brown EW, Macarisin D. 2017. Aeolian contamination of fruits by enteric pathogens:an unexplored paradigm. Curr. Opin. Food Sci. [In Press.] DOI: doi.org/10.1016/j.cofs.2017.12.003
Gao R, Zhu C, Li H, Yin M, Pan C, Huang L, et al. 2017. Dysbiosis signatures of gut microbiota along the sequence from healthy, young patients to those with overweight and obesity. Obesity. 26: 351-361.
Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, et al. 2011. Metagenomic biomarker discovery and explanation. Genome Biol. 12: R60.
Buret AG. 2016. Good bugs, bad bugs in the gut: the role of microbiota dysbiosis in chronic gastrointestinal consequences of infection. Am. J. Gastroenterol. 3: 25-32.
Saha DR, Guin S, Krishnan R, Nag D, Koley H, Shinoda S, et al. 2013. Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies. Gut. Pathog. 5: 36.
Swidsinski A, Loening-Baucke V, Verstraelen H, Osowska S, Doerffel Y. 2008. Biostructure of fecal microbiota in healthy subjects and patients with chronic idiopathic diarrhea. Gastroenterology 135: 568-579.
Albert MJ, Faruque SM, Faruque AS, Neogi PK, Ansaruzzaman M, Bhuiyan NA, et al. 1995. Controlled study of Escherichia coli diarrheal infections in Bangladeshi children. J. Clin. Microbiol. 33: 973-977.
Jin D, Chen C, Li L, Lu S, Li Z, Zhou Z, et al. 2013. Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis. BMC Microbiol. 13: 141.
Segain J, Bletiere DRDL, Bourreille A, Leray V, Gervois N, Rosales C, et al. 2000. Butyrate inhibits inflammatory responses through NFkappaB inhibition: implications for Crohn’s disease. Gut 47: 397-403.
Louis P, Flint HJ. 2009. Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine. FEMS Microbiol. Lett. 294: 1-8.
Xin Y, Gu X, Yin T, Wen H, Gao X, Xi Z. 2015. Study of enteropathogenic bacteria in children with acute diarrhoea aged from 7 to 10 years in Xuzhou, China. Microb. Pathog. 91: 41-45.
Matamoros S, Gras-Leguen C, Le Vacon F, Potel G, de La Cochetiere MF. 2013. Development of intestinal microbiota in infants and its impact on health. Trends Microbiol. 21:167-173.
Palmer C, Bik EM, Digiulio DB, Relman DA, Brown PO. 2007. Development of the human infant intestinal microbiota. PLoS Biol. 5: e177.
Jett BD, Huycke MM, Gilmore MS. 1994. Virulence of enterococci. Clin. Microbiol. Rev. 7: 462-478.
LI ZJ, Rong JR, Zhang SY, Jin D, YE CY, Sun QZ, et al. 2008. Application of 16S rDNA sequence analysis in the bacterial species survey of fecal samples from adult diarrheic patients. Chin. J. Zoonoses 24: 100-103.
LI JZ. 2010. Clinical evaluation with live combined Bacillus subtilis and Enterococcus faecium combined with dimethicone on diarrhea-predominant irritable bowel syndrome. China Med. Herald 5: R574.
Bybee SN, Scorza AV, Lappin MR. 2011. Effect of the probiotic Enterococcus faecium SF68 on presence of diarrhea in cats and dogs housed in an animal shelter. J. Vet. Intern. Med. 25: 856-860.