2018 ; 28(2):
|Author||Daeho Kim, Sanghyun Hong, You-Tae Kim, Sangryeol Ryu, Hyeun Bum Kim, Ju-Hoon Lee|
|Affiliation||Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agricultureand Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea|
|Title||Metagenomic Approach to Identifying Foodborne Pathogens on Chinese Cabbage|
J. Microbiol. Biotechnol.2018 ; 28(2):
|Abstract||Foodborne illness represents a major threat to public health and is frequently attributed to
pathogenic microorganisms on fresh produce. Recurrent outbreaks often come from
vegetables that are grown close to or within the ground. Therefore, the first step to
understanding the public health risk of microorganisms on fresh vegetables is to identify and
describe microbial communities. We investigated the phyllospheres on Chinese cabbage
(Brassica rapa subsp. pekinensis, N = 54). 16S rRNA gene amplicon sequencing targeting the V5-
V6 region of 16S rRNA genes was conducted by employing the Illumina MiSeq system.
Sequence quality was assessed, and phylogenetic assessments were performed using the RDP
classifier implemented in QIIME with a bootstrap cutoff of 80%. Principal coordinate analysis
was performed using a weighted Fast UniFrac matrix. The average number of sequence reads
generated per sample was 34,584. At the phylum level, bacterial communities were composed
primarily of Proteobacteria and Bacteroidetes. The most abundant genera on Chinese cabbages
were Chryseobacterium, Aurantimonadaceae_g, Sphingomonas, and Pseudomonas. Diverse potential
pathogens, such as Pantoea, Erwinia, Klebsiella, Yersinia, Bacillus, Staphylococcus, Salmonella, and
Clostridium were also detected from the samples. Although further epidemiological studies
will be required to determine whether the detected potential pathogens are associated with
foodborne illness, our results imply that a metagenomic approach can be used to detect
pathogenic bacteria on fresh vegetables.|
|Keywords||Phyllosphere, Chinese cabbage, foodborne illness, 16S rRNA gene, bacterial diversity|
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