Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.
Journal of Microbiology and Biotechnology
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2018 ; 28(2): 267~274

AuthorMi-Ae Han, Seong-Joo Hong, Z-Hun Kim, Byung-Kwan Cho, Hookeun Lee, Hyung-Kyoon Choi, Choul-Gyun Lee
AffiliationMarine Bioenergy R&D Center, Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea
TitleEnhanced Production of Fatty Acids via Redirection of Carbon Flux in Marine Microalga Tetraselmis sp.
PublicationInfo J. Microbiol. Biotechnol.2018 ; 28(2): 267~274
AbstractLipids in microalgae are energy-rich compounds and considered as an attractive feedstock for biodiesel production. To redirect carbon flux from competing pathways to the fatty acid synthesis pathway of Tetraselmis sp., we used three types of chemical inhibitors that can block the starch synthesis pathway or photorespiration, under nitrogen-sufficient and nitrogendeficient conditions. The starch synthesis pathway in chloroplasts and the cytosol can be inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 1,2-cyclohexane diamine tetraacetic acid (CDTA), respectively. Degradation of glycine into ammonia during photorespiration was blocked by aminooxyacetate (AOA) to maintain biomass concentration. Inhibition of starch synthesis pathways in the cytosol by CDTA increased fatty acid productivity by 27% under nitrogen deficiency, whereas the blocking of photorespiration in mitochondria by AOA was increased by 35% under nitrogen-sufficient conditions. The results of this study indicate that blocking starch or photorespiration pathways may redirect the carbon flux to fatty acid synthesis.
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KeywordsTetraselmis sp., fatty acids, carbohydrates, nitrogen starvation, chemical inhibitors
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