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 2018 ; 28(2):
267~274
| Author | Mi-Ae Han, Seong-Joo Hong, Z-Hun Kim, Byung-Kwan Cho, Hookeun Lee, Hyung-Kyoon Choi, Choul-Gyun Lee |
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| Affiliation | Marine Bioenergy R&D Center, Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea |
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| Title | Enhanced Production of Fatty Acids via Redirection of Carbon Flux in Marine Microalga Tetraselmis sp. |
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| PublicationInfo |
J. Microbiol. Biotechnol.2018 ; 28(2):
267~274 |
| Abstract | Lipids in microalgae are energy-rich compounds and considered as an attractive feedstock for
biodiesel production. To redirect carbon flux from competing pathways to the fatty acid
synthesis pathway of Tetraselmis sp., we used three types of chemical inhibitors that can block
the starch synthesis pathway or photorespiration, under nitrogen-sufficient and nitrogendeficient
conditions. The starch synthesis pathway in chloroplasts and the cytosol can be
inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 1,2-cyclohexane diamine tetraacetic
acid (CDTA), respectively. Degradation of glycine into ammonia during photorespiration was
blocked by aminooxyacetate (AOA) to maintain biomass concentration. Inhibition of starch
synthesis pathways in the cytosol by CDTA increased fatty acid productivity by 27% under
nitrogen deficiency, whereas the blocking of photorespiration in mitochondria by AOA was
increased by 35% under nitrogen-sufficient conditions. The results of this study indicate that
blocking starch or photorespiration pathways may redirect the carbon flux to fatty acid
synthesis. |
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| Full-Text(PDF) |  |
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| Keywords | Tetraselmis sp., fatty acids, carbohydrates, nitrogen starvation, chemical inhibitors |
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| References |
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