Journal of Microbiology and Biotechnology
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Journal of Microbiology and Biotechnology
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2017 ; 27(6): 1106~1111

AuthorIrisappan Ganesh, Selvamani Vidhya, Gyeong Tae Eom, Soon Ho Hong
AffiliationDepartment of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea
TitleConstruction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System
PublicationInfo J. Microbiol. Biotechnol.2017 ; 27(6): 1106~1111
AbstractEscherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.
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KeywordsChimeric two-component system, Escherichia coli, green fluorescent protein, methanol
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