2017 ; 27(6):
|Author||Irisappan Ganesh, Selvamani Vidhya, Gyeong Tae Eom, Soon Ho Hong|
|Affiliation||Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea|
|Title||Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System|
J. Microbiol. Biotechnol.2017 ; 27(6):
|Abstract||Escherichia coli was engineered to sense methanol by employing a chimeric two-component
system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the
Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and
GFP-based fluorescence analysis showed maximum transcription of ompC and the
fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was
successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using
this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for
the development of biochemical-producing recombinant microorganisms.|
|Keywords||Chimeric two-component system, Escherichia coli, green fluorescent protein, methanol|
Cybulski A. 1994. Liquid-phase methanol synthesis: catalysts, mechanism, kinetics, chemical equilibria, vapor-liquid equilibria, and modeling — a review. Catal. Rev. 36: 557-615.
Arakawa H. 1998. Research and development on new synthetic routes for basic chemicals by catalytic hydrogenation of CO2. Stud. Surf. Sci. Catal. 114: 19-30.
Marchionna M, Girolamo MD, Tagliabue L, Spangler MJ, Fleisch TH. 1998. A review of low-temperature methanol synthesis. Stud. Surf. Sci. Catal. 119: 539-544.
Rozovskii AY, Lin GI. 1999. Catalytic synthesis of methanol. Kinet. Catal. 40: 773-794.
Lange J-P. 2001. Methanol synthesis: a short review of technology improvements. Catal. Today 64: 3-8.
Stock AM, Robinson VL, Goudreau PN. 2000. Two-component signal transduction. Annu. Rev. Biochem. 69: 183-215.
West AH, Stock AM. 2001. Histidine kinases and response regulator proteins in two-component signaling systems. Trends Biochem. Sci. 26: 369-376.
Utsumi R, Brissette RE, Rampersaud A, Forst SA, Oosawa K, Inouye M. 1989. Activation of bacterial porin gene expression by a chimeric signal transducer in response to aspartate. Science 245: 1246-1249.
Weerasuriya S, Schneider BM, Manson MD. 1998. Chimeric chemoreceptors in Escherichia coli: signaling properties of Tar-Tap and Tap-Tar hybrids. J. Bacteriol. 180: 914-920.
Levskaya A, Chevalier AA, Tabor JL, Simpson ZB, Lavery LA, Levy M, et al. 2005. Synthetic biology: engineering Escherichia coli to see light. Nature 438: 441-442.
Ganesh I, Ravikumar S, Lee SH, Park SJ, Hong SH. 2013. Engineered fumarate sensing Escherichia coli based on the novel chimeric two-component system. J. Biotechnol. 168:560-566.
de Vries GE, Harms N, Maurer K, Papendrecht A, Stouthamer AH. 1988. Physiological regulation of Paracoccus denitrificans methanol dehydrogenase synthesis and activity. J. Bacteriol. 170: 3731-3737.
Harms N, Reijnders WNM, Anazawa H, van der Palan CJNM, van Spanning RJM, Oltmann LF, et al. 1993. Identification of a two-component regulatory system controlling methanol dehydrogenase synthesis in Paracoccus denitrificans. Mol. Microbiol. 8: 457-470.
Sambrook J, Russell DW. 2001. Molecular Cloning - A Laboratory Manual. Cold Spring Harbor. Laboratory Press, Cold Spring Harbor, NY. USA.
Baslé A, Rummel G, Storici P, Rosenbusch JP, Schirmer T. 2006. Crystal structure of osmoporin OmpC from E. coli at 2.0 Å. J. Mol. Biol. 362: 933-942.
Eswar N, Webb B, Marti-Renom MA, Madhusudhan MA, Eramian D, Shen MY, et al. 2007. Comparative protein structure modeling using MODELLER. Curr. Protoc. Protein Sci. Chapter 2: Unit 2.9.
Bhattacharya D, Cheng J. 2013. 3Drefine: consistent protein structure refinement by optimizing hydrogen bonding network and atomic-level energy minimization. Proteins 81:119-131.
Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, et al. 1997. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462.
Eleaume H, Jabbouri S. 2004. Comparison of two standardisation methods in real-time quantitative RT-PCR to follow Staphylococcus aureus genes expression during in vitro growth. J. Microbiol. Methods 59: 363-370.
Maruthamuthu M, Ganesh I, Ravikumar S, Hong SH. 2015. Evaluation of zraP gene expression characteristics and construction of a lead (Pb) sensing and removal system in a recombinant Escherichia coli. Biotechnol. Lett. 37: 659-664.
Maruthamuthu MK, Selvamani V, Eom GT, Hong SH. 2017. Development of recA promoter based bisphenol-A sensing and adsorption system by recombinant Escherichia coli. Biochem. Eng. J. 122: 31-37.
Brosnan JT, Brosnan ME. 2006. The sulfur-containing amino acids: an overview. J. Nutr. 136: 1636S-1640S.
Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
Brusstar MJ, Haugen DJ, Gray Jr CL. 2008. Environmental and human health considerations for methanol as a transportation fuel. Presented at the 17th International Symposium on Alternative Fuels, Taiyuan, China, 14 October.
Darmastuti Z, Bhattacharyya P, Andersson M, Kanungo J, Basu S, Käll P, et al. 2013. SiC-FET methanol sensors for process control and leakage detection. Sens. Actuators B Chem. 187: 553-562.