Journal of Microbiology and Biotechnology
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2017 ; 27(3): 429~438

AuthorHercules Sakkas, Chrissanthy Papadopoulou
AffiliationMicrobiology Department, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece
TitleAntimicrobial Activity of Basil, Oregano, and Thyme Essential Oils
PublicationInfo J. Microbiol. Biotechnol.2017 ; 27(3): 429~438
AbstractFor centuries, plants have been used for a wide variety of purposes, from treating infectious diseases to food preservation and perfume production. Presently, the increasing resistance of microorganisms to currently used antimicrobials in combination with the appearance of emerging diseases requires the urgent development of new, more effective drugs. Plants, due to the large biological and structural diversity of their components, constitute a unique and renewable source for the discovery of new antibacterial, antifungal, and antiparasitic compounds. In the present paper, the history, composition, and antimicrobial activities of the basil, oregano, and thyme essential oils are reviewed.
Full-Text(PDF)
KeywordsEssential oils, Thyme, Basil, Antimicrobial activity, Oregano
References
  1. Gurib-Fakim A. 2006. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol. Aspects Med. 27: 1-93.
    Pubmed CrossRef
  2. Prabuseenivasan S, Jayakumar M, Ignacimuthu S. 2006. In vitro antibacterial activity of some plant essential oils. BMC Complement. Altern. Med. 6: 39-50.
    Pubmed CrossRef Pubmed Central
  3. Valiakos E, Marselos M, Sakellaridis N, Constantinidis T, Skaltsa H. 2015. Ethnopharmacological approach to the herbal medicines of the “antidotes” in Nikolaos Myrepsos’ Dynameron. J. Ethnopharmacol. 163: 68-82.
    Pubmed CrossRef
  4. Hammer KA, Carson CF, Riley TV. 1999. Antimicrobial activity of essential oils and other plant extracts. J. Appl. Microbiol. 86: 985-990.
    Pubmed CrossRef
  5. Cowan MM. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12: 564-582.
    Pubmed Pubmed Central
  6. Lardos A. 2006. The botanical material medica of the Iatrosophikon - a collection of prescriptions from a monastery in Cyprus. J. Ethnopharmacol. 104: 387-406.
    Pubmed CrossRef
  7. Malamas M, Marselos M. 1992. The tradition of medicinal plants in Zagori, Epirus (northwestern Greece). J. Ethnopharmacol. 37: 197-203.
    CrossRef
  8. Edris A. 2007. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother. Res. 21: 308-323.
    Pubmed CrossRef
  9. Freires IA, Denny C, Benso B, de Alencar SM, Rosalen PL. 2015. Antibacterial activity of essential oils and their isolated constituents against cariogenic bacteria: a systematic review. Molecules 20: 7329-7358.
    Pubmed CrossRef
  10. Rota C, Carraminana JJ, Burillo J, Herrera A. 2004. In vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne pathogens. J. Food Protect. 67: 1252-1256.
    Pubmed CrossRef
  11. Suhr KI, Nielsen PV. 2003. Antifungal activity of essential oils evaluated by two different application techniques against rye bread spoilage fungi. J. Appl. Microbiol. 94: 665-674.
    Pubmed CrossRef
  12. Burt S. 2004. Essential oils: their antibacterial properties and potential applications in foods - a review. Int. J. Food Microbiol. 94: 223-253.
    Pubmed CrossRef
  13. Pothier J, Galand N, El Ouali M, Viel C. 2001. Comparison of planar chromatographic methods (TLC, OPLC, AMD) applied to essential oils of wild thyme and seven chemotypes of thyme. Il Farmaco 5-7: 505-511.
    CrossRef
  14. Anthony JP, Fyfe L, Smith H. 2005. Plant active components – a resource for antiparasitic agents? Trends Parasitol. 21:462-468.
    Pubmed CrossRef
  15. Aburjai T, Natsheh F. 2003. Plants used in cosmetics. Phytother. Res. 17: 987-1000.
    Pubmed CrossRef
  16. Wallace RJ. 2004. Antimicrobial properties of plant secondary metabolites. Proc. Nutr. Soc. 63: 621-629.
    Pubmed CrossRef
  17. Cooke B, Ernst E. 2000. Aromatherapy: a systematic review. Br. J. Gen. Pract. 50: 493-496.
    Pubmed Pubmed Central
  18. Seymour R. 2003. Additional properties and uses of essential oils. J. Clin. Periodontol. 30: 19-21.
    Pubmed CrossRef
  19. Chorianopoulos N, Kalpoutzakis E, Aligiannis N, Mitaku S, Nychas G-J, Haroutounian S. 2004. Essential oils of Satureja, Origanum and Thymus species: chemical composition and antibacterial activities against foodborne pathogens. J. Agric. Food Chem. 52: 8261-8267.
    Pubmed CrossRef
  20. Cosentino S, Tuberoso SIG, Pisano B, Satta M, Mascia V, Arzedi E, Palmas F. 1999. In vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett. Appl. Microbiol. 29: 130-135.
    Pubmed CrossRef
  21. Holley R, Patel D. 2005. Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiol. 22: 273-292.
    CrossRef
  22. Marino M, Bersani C, Comi G. 2001. Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. Int. J. Food Microbiol. 67: 187-195.
    CrossRef
  23. Smith-Palmer A, Stewart J, Fyfe L. 1998. Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett. Appl. Microbiol. 26: 118-122.
    Pubmed CrossRef
  24. Lang G, Buchbauer G. 2012. A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review. Flavour Fragr. J. 27: 13-39.
    CrossRef
  25. Lambert RJV, Skandamis PN, Coote PJ, Nychas G-JE. 2001. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J. Appl. Microbiol. 91: 453-462.
    Pubmed CrossRef
  26. Guynot ME, Ramos AG, Seto L, Purroy P, Sanchis V, Marin S. 2003. Antifungal activities of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery products. J. Appl. Microbiol. 94: 893-899.
    Pubmed CrossRef
  27. Walsh SE, Maillard JY, Russell AD, Catrenich CE, Charbonneau DL, Bartolo RG. 2003. Activity and mechanisms of action of selected biocidal agents on gram-positive and -negative bacteria. J. Appl. Microbiol. 94: 240-247.
    Pubmed CrossRef
  28. Sikkema J, De Bont J, Poolman B. 1995. Mechanisms of membrane toxicity of hydrocarbons. Microbiol. Rev. 59: 201222.
  29. Si W, Gong J, Tsao R, Zhou T, Yu H, Poppe C, et al. 2006. Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria. J. Appl. Microbiol. 100: 296-305.
    Pubmed CrossRef
  30. Radulovic N, Blagojevic P, Miltojevic A. 2013. α-Linalool - a marker compound of forged/synthetic sweet basil (Ocimum basilicum L.) essential oils. J. Sci. Food Agric. 93:3292-3303.
    Pubmed CrossRef
  31. Rattanachaikunsopon P, Phumkhachorn P. 2010. Antimicrobial activity of basil (Ocimum basilicum) oil against Salmonella enteritidis in vitro and in food. Biosci. Biotechnol. Biochem. 74: 1200-1204.
    Pubmed CrossRef
  32. Suppakul P, Miltz J, Sonneveld K, Bigger S. 2003. Antimicrobial properties of basil and its possible application in food packaging. J. Agric. Food Chem. 51: 3197-3207.
    Pubmed CrossRef
  33. Schulz H, Schrader B, Quilitzsch R, Pfeffer S, Kruger H. 2003. Rapid classification of basil chemotypes by various vibrational spectroscopy methods. J. Agric. Food Chem. 51: 2475-2481.
    Pubmed CrossRef
  34. Trevisan MTS, Silva MGV, Pfundstein B, Spiegelhalder B, Owen RW. 2006. Characterization of the volatile pattern and antioxidant capacity of essential oils from different species of the genus Ocimum. J. Agric. Food Chem. 54: 4378-4382.
    Pubmed CrossRef
  35. Vieira R, Grayer R, Paton A. 2003. Chemical profiling of Ocimum americanum using external flavonoids. Phytochemistry 63: 555-567.
    CrossRef
  36. Tilebeni HG. 2011. Review to basil medicinal plant. Int. J. Agron. Plant Prod. 2: 5-9.
  37. Lachowicz K J, J ones G P, B rig g s DR, Bienvenu F E, W an J , Wilcock A, Coventry MJ. 1998. The synergistic preservative effects of the essential oils of sweet basil (Ocimum basilicum L.) against acid-tolerant food microflora. Lett. Appl. Microbiol. 26: 209-214.
    CrossRef
  38. Opalchenova G, Obreshkova D. 2003. Comparative studies on the activity of basil – an essential oil from Ocimum basilicum L. – against multidrug resistant clinical isolates of the genera Staphylococcus, Enterococcus and Pseudomonas by using different test methods. J. Microbiol. Methods 54: 105-110.
    CrossRef
  39. Lewinsohn E, Ziv-Raz I, Dudai N, Tadmor Y, Lastochkin E, Larkov O, et al. 2000. Biosynthesis of estragole and methyleugenol in sweet basil (Ocimum basilicum L.). Developmental and chemotypic association of allylphenol O-methyltransferase activities. Plant Sci. 160: 27-35.
    CrossRef
  40. Villalobos-Pascual MJ, Acosta-Ballesta MC. 2003. Chemical variation in an Ocimum basilicum gerplasm collection and activity of the essential oils on Callosobruchus maculatus. Biochem. Syst. Ecol. 31: 673-679.
    CrossRef
  41. Kathirvel P, Ravi S. 2012. Chemical composition of the essential oil from basil (Ocimum basilicum Linn.) and its in vitro cytotoxicity against HeLa and HEp-2 human cancer cell lines and NIH 3T3 mouse embryonic fibroblasts. Nat. Prod. Res. 26: 1112-1128.
    CrossRef
  42. Matiz G, Osorio M, Camacho F, Atencia M, Herazo J. 2012. Effectiveness of antimicrobial formulations for acne based on orange (Citrus sinensis) and sweet basil (Ocimum basilicum L.) essential oils. Biomedica 32: 125-133.
  43. Karamaouna F, Kimbaris A, Michaelakis A, Papachristos D, Polissiou M, Papatsakona P, Tsora E. 2013. Insecticidal activity of plant essential oils against the Vine Mealybug, Planococcus ficus. J. Insect Sci. 13: 142.
    CrossRef
  44. Dulger B. 2005. An investigation on antimicrobial activity of endemic Origanum solymicum and Origanum bilgeri from Turkey. Afr. J. Tradit. Complement. Altern. Med. 2: 259-263.
    CrossRef
  45. Kokkini S, Karousou R, Dardioti A, Krigas N, Lanaras T. 1997. Autumn essential oils of Greek oregano. Phytochemistry 44: 883-886.
    CrossRef
  46. Gounaris Y, Skoula M, Fournaraki C, Drakakaki G, Makris A. 2002. Comparison of essential oils and genetic relationship of Origanum × intercedens to its parental taxa in the island of Crete. Biochem. Syst. Ecol. 30: 249-258.
    CrossRef
  47. Burt S, Vlielander R, Haagsman H, Veldhuizen E. 2005. Increase in activity of essential oil components carvacrol and thymol against Escherichia coli O157:H7 by addition of food stabilizers. J. Food Prot. 68: 919-926.
    CrossRef
  48. Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou B. 2001. Composition and antimicrobial activity of the essential oils of two Origanum species. J. Agric. Food Chem. 49: 4168-4170.
    CrossRef
  49. de Falco E, Roscigno C, Landolfi S, Scandolera E. 2014. Growth, essential oil characterization, and antimicrobial activity of three wild types of oregano under cultivation condition in Southern Italy. Ind. Crops Prod. 62: 242-249.
    CrossRef
  50. Baydar H, Sagdic O, Ozkan G, Karadogan T. 2003. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control 15: 169-172.
    CrossRef
  51. Alma M, Mavi A, Yildirim A, Digrak M, Hirata T. 2003. Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biol. Pharm. Bull. 26: 1725-1729.
    CrossRef
  52. Sagdic O. 2003. Sensitivity of four pathogenic bacteria to Turkish thyme and oregano hydrosols. LWT Food Sci. Technol. 36: 467-473.
    CrossRef
  53. Vagi E, Simandi B, Suhajda A, Hethelyi E. 2005. Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and supercritical carbon dioxide. Food Res. Int. 38: 51-57.
    CrossRef
  54. Nostro A, Blanco A, Cannatelli M, Enea V, Flamini G, Morelli I, et al. 2004. Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbiol. Lett. 230: 191-195.
    CrossRef
  55. McKay D, Blumberg J. 2006. A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.). Phytother. Res. 20: 519-530.
    CrossRef
  56. Cetin H, Cilek J, Aydin L, Yanikoglu A. 2009. Acaricidal effects of the essential oil of Origanum minutiflorum (Lamiaceae) against Rhipicephalus turanicus(Acari: Ixodidae). Vet. Parasitol. 160: 359-361.
    CrossRef
  57. Mith H, Dure R, Delcenserie V, Zhiri A, Daube G, Clinquart A. 2014. Antimicrobial activities of commercial essential oils and their components against food-borne pathogens and food spoilage bacteria. Food Sci. Nutr. 2: 403-416.
    CrossRef
  58. Nabavi SM, Marchese A, Izadi M, Curti V, Daglia M, Nabavi SF. 2015. Plants belonging to the genous Thymus as antibacterial agents: from farm to pharmacy. Food Chem. 173:339-347.
    CrossRef
  59. Badi HN, Yazdani D, Ali SM, Nazari F. 2004. Effects of spacing and harvesting time on herbage yield and quality/quantity of oil in thyme, Thymus vulgaris L. Ind. Crop. Prod. 19: 231-236.
    CrossRef
  60. Senatore F. 1996. Influence of harvesting time on yield and composition of the essential oil of a thyme (Thymus pulegioides L.) in Campania (Southern Italy) J. Agric. Food Chem. 44:1327-1332.
    CrossRef
  61. Guillen MD, Manzanos MJ. 1998. Study of the composition of the different parts of a Spanish Thymus vulgaris L. plant. Food Chem. 63: 373-383.
    CrossRef
  62. Ben-Jabeur M, Ghabri E, Myriam M, Hamada W. 2015. Thyme essential oil as a defense inducer of tomato against gray molf and Fusarium wilt. Plant Physiol. Biochem. 94: 35-40.
    CrossRef
  63. Rasooli I, Rezaei MB, Allameh A. 2006. Ultrastructural studies on antimicrobial efficacy of thyme essential oils on Listeria monocytogenes. Int. J. Infect. Dis. 10: 236-241.
    CrossRef
  64. Schulz H, Quilitzsch R, Kruger H. 2003. Rapid evaluation and quantitative analysis of thyme, origano and chamomile essential oils by ATR-IR and NIR spectroscopy. J. Mol. Struct. 661-662: 299-306.
    CrossRef
  65. Yamazaki K, Yamamoto T, Kawai Y, Inoue N. 2004. Enhancement of antilisterial activity of essential oil constituents by nisin and diglycerol fatty acid ester. Food Microbiol. 21: 283-289.
    CrossRef
  66. Burt SA, Reinders RD. 2003. Antibacterial activity of selected plant essential oils against Escherichia coli O157-H7. Lett. Appl. Microbiol. 36: 162-167.
    CrossRef
  67. Valero M, Salmeron MC. 2003. Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. Int. J. Food Microbiol. 85: 73-81.
    CrossRef
  68. Saccheti G, Medici A, Maietti S, Radice M, Muzzoli M, Manfredini S, et al. 2004. Composition and functional properties of the essential oil of Amazonian Basil, Ocimum micranthum Willd., Labiatae in comparison with commercial essential oils. J. Agric. Food Chem. 52: 3486-3491.
    CrossRef
  69. Sokmen M, Serkedjieva J, Daferera D, Gulluce M, Polissiou M, Tepe B, et al. 2004. In vitro antioxidant, antimicrobial and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. J. Agric. Food Chem. 52: 3309-3312.
    CrossRef
  70. Bozin B, Dukic-Mimica N, Simin N, Anackov G. 2006. Characterization of the volatile composition of essential oils of some Lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils. J. Agric. Food Chem. 54: 1822-1828.
    CrossRef
  71. Schelz Z, Molnar J, Hohmann J. 2006. Antimicrobial and antiplasmid activities of essential oils. Fitoterapia 77: 279-285.
    CrossRef
  72. Sakkas H, Gousia P, Economou V, Sakkas V, Petsios S, Papadopoulou C. 2016. In vitro antimicrobial activity of five essential oils on multidrug resistant gram-negative clinical isolates. J. Intercult. Ethnopharmacol. 5: 212-218.
    CrossRef
  73. Cavaleiro C, Pinto E, Goncalves MJ, Salgueiro L. 2006. Antifungal activity of Juniperus against dermatophyte, Aspergillus and Candida strains. J. Appl. Microbiol. 100: 1333-1338.
    CrossRef
  74. Giordani R, Regli P, Kaloustian J, Mikail C, Abou L, Portugal H. 2004. Antifungal effect of various essential oils against Candida albicans. Potentiation of antifungal action of amphotericin B by essential oil from Thymus vulgaris. Phytother. Res. 18: 990-995.
    CrossRef
  75. Tampieri MP, Galuppi R, Macchioni F, Carelle MS, Falcioni L, Cioni PL, Morelli I. 2005. The inhibition of Candida albicans by selected essential oils and their major components. Mycopathologia 159: 339-345.
    CrossRef
  76. Pina-Vaz C, Goncalves-Rodrigues A, Pinto E, Costa-de Oliveira S, Tavares C, Salgueiro L, et al. 2004. Antifungal activity of thymus oils and their major components. J. Eur. Acad. Dermatol. Venereol. 18: 73-78.
    CrossRef
  77. Salgueiro LR, Cavaleiro C, Pinto E, Pina-Vaz C, Rodrigues AG, Palmeira A, et al. 2003. Chemical composition and antifungal activity of the essential oil of Origanum virens on Candida species. Planta Med. 69: 871-874.
    CrossRef
  78. Pinto E, Pina-Vaz C, Salgueiro L, Goncalves MJ, Costa-de Oliveira S, Cavaleiro C, et al. 2006. Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species. J. Med. Microbiol. 55: 1367-1373.
    CrossRef
  79. Soliman KM, Badeaa RI. 2002. Effect of oil extracted from some medicinal plants of different mycotoxigenic fungi. Food Chem. Toxicol. 40: 1669-1675.
    CrossRef
  80. Sakkas H, Gousia P, Economou V, Petsios S, Papadopoulou C. 2016. Antifungal activity of four essential oils against Candida clinical isolates. Asian J. Ethnopharmacol. Med. Foods 2: 22-25.
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